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MYO18B通过促进焦点粘附成熟促进溶酶体外细胞分裂
Wei-Wei Ren1,2, Rebeca Kawahara3, Kenichi G N Suzuki2,4
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
The Journal of cell biology
|January 3, 2025
概括
癌细胞通过溶酶体外细胞分裂在焦点粘附附近释放少量曼诺基基蛋白质. MYO18B和PIEZO1促进了这一过程,将 lysosomal 功能与细胞机械感知联系起来.
科学领域:
- 细胞生物学 细胞生物学
- 葡萄糖生物学 葡萄糖生物学
- 癌症研究 癌症研究
背景情况:
- 癌细胞往往表现出较高的paucimannose glycans,截断的N-glycans在健康哺乳动物中不常见.
- 源自 lysosomes 的缺乏曼诺酸蛋白的细胞表面暴露的机制仍然不清楚.
研究的目的:
- 阐明癌细胞从癌细胞中分泌的缺乏曼诺酸蛋白质的机制.
- 为了研究焦点粘附和机械感应在溶酶体外细胞形成中的作用.
主要方法:
- lysosomal glycosidase 的活性测定方法.
- lysosomal exocytosis 追踪 追踪 追踪 追踪 追踪 追踪 追踪
- 全基因组淘汰查 (确定MYO18B)
- 成像 (PIEZO1激活) 的使用
主要成果:
- 帕乌西曼诺基的蛋白质是由 lysosomal glycosidases 生产的,并通过 lysosomal exocytosis 分泌出来.
- lysosomal exocytosis 在空间上与焦点粘附有关.
- MYO18B对于焦点粘附成熟和随后的溶酶体外细胞形成至关重要.
- 在焦点粘附处的机械敏感通道PIEZO1调节了Ca2+的流入,用于溶解体-等离子体膜融合.
结论:
- lysosomal exocytosis 是一个关键的途径,用于释放癌细胞中的 paucimannosidic 蛋白.
- 焦点粘附和actin细胞骨在调节 lysosomal exocytosis 中起着至关重要的作用.
- 通过PIEZO1,细胞机械感知与 lysosomal exocytosis 集成,揭示了一个新的生物途径.
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