基于色度测量和表面增强的拉曼散射,用于测量四环素抗生素的竞争性双模式
Junjie Qi1, Yuqi Wan1, Jingkun Li1
1State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China; International Joint Laboratory on Food Safety, Jiangnan University, 214122, Wuxi, Jiangsu, People's Republic of China.
Biosensors & bioelectronics
|January 3, 2025
概括
使用色度检测和表面增强拉曼散射 (SERS) 的新双模式检测方法可在食品和环境样本中对四环素抗生素 (TCs) 进行敏感和快速评估.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 环境科学 环境科学
背景情况:
- 四环素抗生素 (TCs) 广泛使用,但它们的过度使用会导致食物和环境中的积累.
- 由于抗菌素耐药性,这种积累对人类健康构成重大威胁.
- 现有的检测方法可能缺乏有效监测所需的灵敏度或速度.
研究的目的:
- 开发一种新的双模式检测方法,用于对TC的敏感和快速评估.
- 将色度传感与表面增强拉曼散射 (SERS) 技术集成在一起.
- 为了创建一个纳米探测器,增强催化和SERS特性用于TCs检测.
主要方法:
- 一个Ag@NH2-MIL-101 ((Al) -Ag纳米探针的设计和合成.
- 利用纳米探针的过氧化酶类活性,与TMB进行竞争性色度测试.
- 采用纳米探针增强的SERS性能用于敏感TC检测.
- 使用蜂蜜样本验证方法.
主要成果:
- 双模式方法实现了对TCs的高灵敏度和选择性.
- 检测极限 (LODs) 是10−3 ppm (色度计) 和10−5 ppm (SERS).
- 在蜂蜜样本中显示可靠的检测,恢复率从87.69%到120.49%.
结论:
- 开发的色度测量-SERS双模式传感策略为TC检测提供了一种有价值的工具.
- 这种方法将色度分析的易用性与SERS的高灵敏度相结合.
- 它为环境和食品链中的各种危险提供了快速,直接和多重评估.
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