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OsNCED5通过调节大米中的ROS恒常性来赋予寒冷应激耐受性

Zhipan Xiang¹, Lin Zhang², Mingze Zhang³

¹School of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun, 558000, China; Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.

Plant physiology and biochemistry : PPB

|January 3, 2025

概括

米植物米植物

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

寒冷压力对大米的生长和产量产生重大影响.
酸 (ABA) 对于植物应激反应至关重要,但其在米寒冷耐受性中的作用尚不清楚.

研究的目的:

识别和描述涉及酸 (ABA) 介导的米冷应激耐受性的基因.
研究OsNCED5在调节寒冷耐受性方面的功能.

主要方法:

通过CRISPR/Cas9介导的突变发生来产生OsNCED5淘汰米.
对酸 (ABA) 含量的定量分析.
在幼苗阶段评估寒冷应激耐受性.
基因表达分析和OsNCED5.5的亚细胞局部化.

主要成果:

OsNCED5是一种ABA生物合成酶,由大米的冷应激诱导.
OsNCED5的干扰降低了ABA水平和耐寒性;外源性ABA拯救了表型.
OsNCED5过度表达增强了大米的寒冷耐受性.
OsNCED5通过维持活性氧物种 (ROS) 恒温来调节寒冷耐受性.

结论:

OsNCED5是大米耐寒压力的关键调节剂,通过ABA生物合成和ROS恒温作用.
OsNCED5代表了一种改善大米耐寒性的潜在遗传标.

关键词:

9-cis-epoxycarotenoid二氧化碳酶的使用方法阿巴阿巴阿巴是什么意思耐寒压力耐受性耐寒压力耐受性有反应性氧物种的反应性氧物种.大米大米大米大米大米

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