相关实验视频

Updated: Apr 30, 2026

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Published on: September 13, 2012

超声波组合pH转移策略,以提高酵母蛋白稳定乳液的稳定性:专注于溶解性,蛋白质结构,接口特性

Xueting Zheng¹, Tianfu Cheng¹, Sibo Liu¹

¹College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.

International journal of biological macromolecules

|January 3, 2025

概括

结合的超声波和pH转移处理显著提高酵母蛋白 (YP) 溶解度,改善乳液稳定性. 这种新的方法优化了YP的功能,用于食品应用.

科学领域:

食品科学食品科学食品科学
蛋白质化学蛋白质化学
体科学体科学体科学

背景情况:

酵母蛋白 (YPs) 是食品系统中有价值的功能成分.
乳液稳定性对于产品质量和保质期至关重要.
优化YP功能需要了解它们的结构和界面行为.

研究的目的:

为了研究结合超声波和pH转移处理改善YP功能的机制.
阐明这些治疗方法如何影响YP的溶解性,结构和界面特性.
评估对乳液稳定性和滴滴特性的影响.

主要方法:

用超声波和pH转移 (pH-U) 联合处理YP.
测量YP的溶解性,二级/三级结构 (β-片,α-螺旋,二硫化键,光),以及表面的疏水性.
对界面特性 (张力,扩散,透,重组,度) 的分析.
评估乳化特性 (活性和稳定性指数) 和乳液滴滴大小/均性.
使用多重光散射评估乳液稳定性的评估.

主要成果:

结合pH-U处理显著增加了YP溶解度,pH-U400达到85.51%.
蛋白质结构分析揭示了二次结构,二硫化键,光强度和表面水性的变化.
pH-U400表现出较低的界面张力和增强的界面扩散,透和重组.
pH-U400显示出优异的乳化特性 (EAI: 27.05 m2/g,ESI: 31.27 分钟),并产生较小,均的乳液滴.
经pH-U400稳定的乳液显示出最高的稳定性.

结论:

结合超声波和pH转移治疗有效地修改YP结构并增强它们的界面行为.
这种优化导致了显著改善的乳液稳定能力.
这些发现为YP稳定乳液的机制提供了洞察力,并指导了YP产品用于食品工业的开发.

关键词:

接口行为接口行为蛋白质的结构蛋白质的结构.可溶性溶解度可溶性溶解度超声波超声波的使用方法酵母蛋白是酵母蛋白中的一种蛋白质.改变pH值的变化

更多相关视频

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

相关概念视频

Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

The Colloidal State

The Colloidal State

The formation of a colloidal system is exemplified by an aqueous solution containing Cl− ions is introduced to another containing Ag+ ions, resulting in the precipitation of solid AgCl as extremely tiny crystals. Instead of settling out as a filterable precipitate, these crystals remain suspended in the liquid, showcasing a colloidal system.A colloidal system involves colloidal particles within the approximate range of 1 to 1000 nm in at least one dimension, dispersed in a medium called...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...