Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

VSEPR Theory and the Basic Shapes02:52

VSEPR Theory and the Basic Shapes

67.4K
Overview of VSEPR Theory
67.4K
VSEPR Theory02:37

VSEPR Theory

9.0K
Valence shell electron-pair repulsion theory (VSEPR theory) enables us to predict the molecular structure around a central atom from an examination of the number of bonds and lone electron pairs in its Lewis structure. The VSEPR model assumes that electron pairs in the valence shell of a central atom will adopt an arrangement that minimizes repulsions between these electron pairs by maximizing the distance between them. The electrons in the valence shell of a central atom form either bonding...
9.0K
Van der Waals Interactions01:24

Van der Waals Interactions

63.5K
Atoms and molecules interact with each other through intermolecular forces. These electrostatic forces arise from attractive or repulsive interactions between particles with permanent, partial, or temporary charges. The intermolecular forces between neutral atoms and molecules are ion–dipole, dipole–dipole, and dispersion forces, collectively known as van der Waals forces.
63.5K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

48.3K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
48.3K
Valence Bond Theory02:42

Valence Bond Theory

8.4K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
8.4K
Position-effect Variegation02:32

Position-effect Variegation

6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structural basis for multivitamin recognition and transport by human SMVT.

Nature communications·2026
Same author

Pericentrosomal Redistribution of the Endoplasmic Reticulum Ensures Organelle Symmetric Inheritance and Mitotic Progression.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

CCDC120 phase separation contributes to desmosomal integrity and cardiac function.

Nature communications·2026
Same author

Acetylation of STX17 promotes its autophagosomal translocation.

Nature communications·2026
Same author

Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation.

Nature communications·2026
Same author

Hyperglycemia promotes SIRT3-mediated deacetylation of SARM1 to exacerbate diabetic peripheral neuropathy in mice.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Jun 4, 2025

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function
09:09

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

Published on: August 7, 2019

6.0K

人类VANGL-PRICKLE相互作用的结构基础

Yanyi Song1, Shuyi Jian2, Junlin Teng2

  • 1State Key Laboratory of Membrane Biology, Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.

Nature communications
|January 3, 2025
PubMed
概括

这项研究揭示了高样 (VANGL) - - 皮克尔 (PK) 相互作用的结构基础,这对于平面细胞极性 (PCP) 至关重要. 研究人员发现了PK1如何将含有VANGL的囊泡引导到细胞膜,从而推进了对PCP通路的理解.

更多相关视频

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.1K
Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
11:56

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Published on: November 9, 2009

21.3K

相关实验视频

Last Updated: Jun 4, 2025

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function
09:09

Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function

Published on: August 7, 2019

6.0K
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

11.1K
Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle
11:56

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Published on: November 9, 2009

21.3K

科学领域:

  • 细胞生物学 细胞生物学
  • 发展生物学 发展生物学
  • 结构生物学 结构生物学

背景情况:

  • 平面细胞极性 (PCP) 控制着细胞和组织在发育过程中的组织.
  • - (PK) 蛋白复合体是PCP的关键调节者,但其结构细节和功能在很大程度上仍然未知.
  • 了解PCP的分子机制对于发育生物学和疾病研究至关重要.

研究的目的:

  • 阐明VANGL蛋白和PRICKLE蛋白之间的相互作用的结构基础.
  • 研究PRICKLE1在细胞内VANGL蛋白的局部化中的功能性作用.
  • 提供对平面细胞极性调节的分子洞察力.

主要方法:

  • 使用冷电子显微镜 (cryo-EM) 用PRICKLE1.1确定人类VANGL1,VANGL2及其复合物的高分辨率结构.
  • 生物化学试验被用来分析VANGL-PK相互作用.
  • 进行了细胞成像实验,以可视化蛋白质的定位和功能.

主要成果:

  • 人类VANGL1,VANGL2和VANGL-PRICKLE1复合体的5个冷EM结构在2.2-3.0 Å分辨率下得到分辨.
  • 已经破译了VANGL-PK相互作用的分子细节.
  • 已经证明PRICKLE1能够将含有VANGL的细胞内囊泡向外围细胞膜.

结论:

  • 这项研究提供了首次对VANGL-PK相互作用的高分辨率结构洞察,这是PCP途径的关键组成部分.
  • PRICKLE1在贩运VANGL蛋白到细胞外围方面发挥着直接作用,影响PCP的建立.
  • 这些发现为进一步研究PCP的分子机制和相关的发育过程提供了基础.