使用来自同一阅读的核和叶绿体数据进行亚家族进化分析
Eranga Pawani Witharana1,2,3, Takaya Iwasaki4, Myat Htoo San5,6
1Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka. erangav8@gmail.com.
Scientific reports
|January 3, 2025
概括
使用相同的原始测序数据进行的叶绿体 (cp) 和核DNA分析揭示了植物的进化关系. 不完整的谱系排序和内向解释了cp基因组和Aurantioideae.核DNA树之间的差异.
科学领域:
- 进化生物学 进化生物学
- 基因组学就是基因组学.
- 人类遗传学 是一个学科.
背景情况:
- 叶绿体 (cp) 基因组对于植物进化研究有价值,但它们的分辨率有时是有限的.
- 将cp基因组数据与核DNA集成,可以提供更完整的进化图像,但通常需要单独的数据集.
- 包括类在内的Aurantioideae亚家族呈现出复杂的进化关系.
研究的目的:
- 开发一种成本效益高的方法,利用Read2Tree从原始测序数据中提取核基因序列.
- 从相同的原始测序数据中构建和比较基于cp基因组和核DNA的家族遗传树.
- 调查cp和Aurantioideae中的基于核DNA的家族遗传树之间的不一致的原因.
主要方法:
- 利用Read2Tree从原始测序数据中提取保存的核基因序列.
- 使用cp基因组数据和核DNA序列构建了家族遗传树.
- 采用具有多个基因对齐的整合性工作流程和族系学方法来分析不一致性.
主要成果:
- 来自Read2Tree的核DNA树与现有的核家族相一致.
- 在基于cp基因组的树和基于核DNA的树之间观察到显著的差异.
- 不完整的血统分类被确定为不一致的主要驱动因素,内进也有所贡献.
结论:
- Read2Tree提供了一种具有成本效益的方法,用于从原始测序数据中进行核DNA遗传学分析.
- 在Aurantioideae中cp和核DNA族系之间的不一致主要是由于不完整的血统分类和内向.
- 在CP和核DNA分析中使用相同的原始测序数据可以提高对植物进化关系的理解.
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