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暴露于童年虐待与精子中的特定表观遗传模式有关

Jetro J Tuulari^1,2,3,4, Matthieu Bourgery^5,6, Jo Iversen⁷

¹FinnBrain Birth Cohort Study, Turku Brain and Mind Center, Department of Clinical Medicine, University of Turku, Turku, Finland. jetro.tuulari@utu.fi.

Molecular psychiatry

|January 3, 2025

概括

儿童虐待暴露 (CME) 可以在表观遗传上改变精子,可能会影响后代的健康. 这项研究发现,在有CME史的男性中,精子DNA甲基化和小RNA的差异.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
生殖生物学生殖生物学
发育神经科学的发展神经科学.

背景情况:

儿童虐待 (CME) 暴露与不良健康结果有关.
动物研究表明,生殖线表观遗传变化会传递代际效应.
人类研究是必要的,以探索在早期生活压力后的父亲生殖系变化.

研究的目的:

调查父亲CME与人类精子表观基因组之间的关联.
识别与CME相关的精子小非编码RNA (sncRNA) 和DNA甲基化 (DNAme) 的变化.
探索对后代神经发育的潜在代际影响.

主要方法:

使用FinnBrain出生队列进行的病例控制研究.
父亲CME通过创伤和痛苦量表 (TADS) 进行评估.
使用小RNA测序 (小RNA-seq) 分析的精子sncRNA.
精子DNA甲基化映射使用减少表示双硫酸盐测序 (RRBS-seq).

主要成果:

在高和低TADS组之间确定了3个不同的甲基化基因组区域.
检测到68种差异表达的sncRNA,包括tRNA衍生小RNA (tsRNA) 和miRNA.
在CRTC1和GBX2基因附近发现miRNA hsa-mir-34c-5p的差异表达和甲基化变化.

结论:

早期的生活压力会影响父亲的生殖系表观基因组.
精子表观遗传修饰可能是CME代际影响的机制.
这些发现支持其在调节后代中枢神经系统发育方面的潜在作用.

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