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精选的Lactobacillus acidophilus NCFM基因组提供了对菌株特异性和微观进化的见解

Meichen Pan¹, Sarah O'Flaherty¹, Ashley Hibberd²

¹Department of Food, Bioprocessing, & Nutrition Sciences, North Carolina State University, Raleigh, NC, USA.

|January 3, 2025

概括

这项研究分析了114个Lactobacillus acidophilus基因组,揭示了NCFM菌株的高同质性和基因组稳定性. 这证实了它作为益生菌和生物治疗药物的适用性.

科学领域:

微生物学微生物学
基因组学就是基因组学.
细菌进化的过程

背景情况:

下一代测序已经增加了细菌基因组数据,提高了对基因组组成和进化的理解.
乳酸菌 Lactobacillus acidophilus NCFM 是一个商业上重要的益生菌细菌,在食品和治疗中使用了数十年.
研究的重点是工业细菌,包括乳酸菌,因为它们的益生菌应用.

研究的目的:

为了重新评估20年前的Lactobacillus acidophilus基因组序列.
确定114种公开可用的L. acidophilus菌株的核心和泛基因组.
研究L. acidophilus NCFM衍生变体中的基因组稳定性和微观进化.

主要方法:

114种Lactobacillus acidophilus菌株的比较基因组学.
核心和泛基因组的分析.
对21种L. acidophilusNCFM衍生的变种进行基因组稳定性的检查.

主要成果:

在L. acidophilus物种中观察到高同质性,在现有的基因组序列中发现了一些错误组合.
在L. acidophilus NCFM变体中证明了基因组稳定性,没有观察到重排或反转.
随着时间的推移和在不同的测序平台上,在NCFM衍生菌株中发现的最小单核酸多态 (SNPs).

结论:

对比基因组分析支持可用的L. acidophilus基因组的可靠性.
L. acidophilus NCFM表现出显著的基因组稳定性,验证了其作为益生菌的使用.
该NCFM菌株的遗传完整性支持其作为新型生物治疗药物的工程底盘的作用.

关键词:

乳杆菌酸性动物NCFM NCFM 进行比较的基因组学.基因组基因组基因组基因组基因组潘基因基因组是如何形成的益生菌是一种益生菌.单个核酸的多态性.菌株变化的变化

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