多基因 Mycobacterium tuberculosis 无细胞DNA测定方法
S Ayalew1, T Wegayehu2, B Wondale2
1Armauer Hansen Research Institute, Addis Ababa, Ethiopia;, Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia.
概括
一个新的结核病 (TB) 血DNA测试显示出有希望的结果. 从结核病患者的无细胞DNA (cfDNA) 中准多个基因可以提高诊断灵敏度,为基于唾液的方法提供潜在的替代方案.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 目前的结核病诊断通常依赖于唾液,这带来了收集挑战.
- 菌结核无细胞DNA (Mtb cfDNA) 提供了一个替代的诊断点.
研究的目的:
- 评估使用血Mtb cfDNA进行定量PCR (qPCR) 试验以诊断肺结核.
- 评估针对多个基因的敏感性和特异性 (IS6110,cyp141,devR).
主要方法:
- 分析了来自106名肺结核患者和60名对照组的血样本.
- 一个qPCR测定针对IS6110,cyp141和devR基因.
- 敏感性与Xpert MTB/RIF,培养和临床诊断进行了比较;特异性与对照进行了比较.
主要成果:
- Mtb cfDNA测定在所有PTB病例中获得了71.7%的灵敏度,在细菌学上确诊的病例中 (78.3%) 比临床诊断的病例 (28.6%) 高.
- 综合的特异性为91.7%.
- 针对多个基因,特别是IS6110和cyp141 (70.8%的灵敏度),与单基因目标相比,提高了总体检测率.
结论:
- 血Mtb cfDNA定量PCR是一种潜在的结核病诊断辅助工具.
- 多重基因向提高了基于cfDNA的结核病诊断的灵敏度.
- 这种方法可以克服与唾液样本采集相关的局限性.
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