低位性压力会改变昼夜基因的转录
Androniqi Qifti1, Ayobami Adeeko1, Madison Rennie1
1Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, Massachusetts.
Biophysical journal
|January 4, 2025
概括
低位性压力通过影响Bmal1局部化,暂时停止细胞周期和昼夜节律. 这种干扰与洞穴变形和cavin-1释放有关,影响基因转录.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生理学 生理学 生理学
背景情况:
- 细胞通过胀,氧化物增加和基因转录来适应低氧化应激.
- 已知循环节律基因对细胞条件敏感.
研究的目的:
- 在轻微的低度压力下调查基因表达变化.
- 确定透应激和昼夜节律调节之间的联系.
- 阐明透应激影响细胞循环进展的机制.
主要方法:
- 培养的老鼠光滑肌细胞 (WKO-3M22) 暴露在轻微的低体应激中12小时和24小时.
- 对基因转录转移的分析.
- 观察Bmal1 (一个关键的昼夜转录因子) 的局部化.
- 研究cavin-1 (一个洞穴结构蛋白) 的作用和洞穴表达水平.
主要成果:
- 低位性压力诱导了与昼夜节律相关的基因 (per1, nr1d1, per2, dbp, Ciart) 转录的变化.
- 在12小时的压力后,Bmal1的核定位被破坏 (细胞质定位),与细胞循环停止相关.
- 24小时后Bmal1的核定位恢复,细胞循环恢复.
- 低调cavin-1或减少洞穴表达使Bmal1局部化独立于透应激.
结论:
- 透应激可以暂时停止昼夜节律和细胞循环的进展.
- 洞穴变形和随后的cavin-1释放似乎通过影响Bmal1核转位和基因转录来调解这种效应.
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