交生体Wolbachia通过调节宿主排毒功能,增加了Ectropis grisescens中对双林的抵抗力
Tian Gao1, Yong Zhang2, Wanpeng Sun2
1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China; College of Horticulture, Northwest A＆F University, Yangling, Shaanxi, China.
Ecotoxicology and environmental safety
|January 4, 2025
概括
昆虫共生体,特别是Wolbachia,可能会降低Ectropis grisescens对双林的抵抗力. 通过使用四环素来削减Wolbachia,降低了害虫的生存率,并通过影响细胞染色体P450基因增加了对杀虫剂的敏感性.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 微生物生态学 微生物生态学
- 虫害管理 虫害管理 虫害管理
背景情况:
- 像Ectropis grisescens这样的害虫对杀虫剂的耐药性是一个重要的农业挑战.
- 昆虫共生体在调解杀虫剂耐药性的作用是一个新兴的研究领域.
- 对共生体对E. grisescens抗昆虫剂耐药性的具体贡献仍然在很大程度上是未知的.
研究的目的:
- 调查昆虫共生体,特别是Wolbachia在Ectropis grisescens对杀虫剂双林的耐药性方面的潜在作用.
- 探索共生体操纵对E. grisescens杀虫剂易感性和排毒机制的影响.
主要方法:
- 使用抗生素治疗 (四环素,安西林,珍塔素,链杆菌素) 来选择性地消除或减少E. grisescens.中的共生动物种群.
- 进行了生物测试,以评估昆虫杀虫剂敏感性 (二丁) 和生存率的变化.
- 酶活性测定 (P450) 和分子技术 (16S rRNA测序,转录组分析) 用于调查潜在机制.
主要成果:
- 抗生素治疗,特别是四环素治疗,显著增加了E. grisescens对双林的敏感性.
- 环素治疗以剂量依赖的方式降低了沃尔巴基亚的存在,P450酶活性和生存率.
- 沃尔巴基亚的枯竭导致了关键的细胞染色体P450基因的下调,这些基因参与了异生物代谢.
结论:
- 沃尔巴基亚似乎在赋予E. grisescens对双氨酸耐药性的过程中发挥着至关重要的作用.
- 该机制涉及沃尔巴基亚对细胞染色体P450基因的调节,影响害虫的排毒能力.
- 这些发现为开发针对共生体的新型害虫防治策略对抗抗性昆虫种群提供了基础.
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