在未成熟的卵细胞复合体中化激素受体缺乏,用于辅助生殖
Maíra Casalechi1, Maria Thereza V Pereira1, Wiviane A Assis1
1Division of Human Reproduction, Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
F&S science
|January 4, 2025
概括
化激素受体 (LHR) 的表达在不成熟卵细胞的粒状细胞中较低. 这种缺陷可能解释了为什么在辅助生殖技术过程中检索胚芽囊泡阶段卵细胞.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 细胞生物学 细胞生物学
- 辅助生殖技术 辅助生殖技术
背景情况:
- 颗粒状细胞在卵细胞成熟中起着至关重要的作用.
- 化激素受体 (LHR) 对于卵泡发育和排卵至关重要.
- 了解LHR表达动态对于改善辅助生殖技术 (ART) 结果至关重要.
研究的目的:
- 为了研究黄素化激素受体 (LHR) 在颗粒细胞表达和卵细胞成熟阶段之间的关系.
- 确定LHR缺陷是否有助于ART中未成熟卵细胞的检索.
主要方法:
- 对LHR信使核糖核酸 (mRNA) 和人类毛囊中的颗粒细胞中的蛋白质表达的分析.
- LHR表达水平与卵细胞成熟阶段的相关性 (胚芽囊与成熟).
- 在体外成熟 (IVM) 救援实验以评估卵细胞的发育潜力.
主要成果:
- 检测到LHR mRNA和蛋白质表达在围绕生殖囊阶段卵细胞的积分细胞中很少或不存在.
- 在生殖囊泡阶段被捕的卵细胞在通过IVM获救时显示出成熟的潜力.
- 实体细胞LHR缺乏被确定为在ART期间检索不成熟卵细胞的潜在因素.
结论:
- 化激素受体 (LHR) 的表达在未成熟卵细胞的粒状细胞中明显较低.
- 在体细胞中LHR缺乏可能是ART中取回生殖囊泡阶段卵细胞的主要原因.
- 向LHR表达可能会提高卵细胞质量和ART成功率.
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