鉴定和描述ClAPRR2,一个关键的候选基因控制西瓜条纹颜色的关键候选基因
Shuang Liang1, Miaomiao Yang1, Linlin Zhang1
1Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
遗传研究发现APRR2基因对西瓜条纹颜色至关重要. 这种基因的变异,特别是在父母中的一个插入,可能决定西瓜的条纹图案.
科学领域:
- 植物遗传学 植物遗传学
- 提高作物质量 提高作物质量
- 园艺科学 园艺科学
背景情况:
- 瓜子的条纹颜色是一个关键的商业特征,但它的遗传基础仍然不清楚.
- 了解条纹颜色遗传学可以帮助开发改进的西瓜品种.
研究的目的:
- 为了精确地绘制和识别负责西瓜条纹颜色的基因.
- 为了阐明底层条纹颜色变化的分子机制.
主要方法:
- 构建一个大F2种群 (710种植物) 来自对比的亲系.
- 精细映射,基因测序,叶绿素含量分析,亚细胞定位和促进体分析.
主要成果:
- 条纹色基因被定位在9号染色体上的85.284kb区域,其中包含五个候选基因.
- Cla97C09G175170 (APRR2) 被确定为最可能的候选基因.
- 在APRR2中插入浅绿色条纹母体 (ZXG1555) 的12bp插入导致了保留域的过早终止和删除,可能会影响蛋白质功能和定位.
结论:
- APRR2是控制西瓜条纹颜色的主要基因.
- 遗传变异,包括APRR2的编码和促进区域的插入和删除,有助于条纹颜色差异.
- 鉴定的变异为旨在控制西瓜条纹模式的育种计划提供了洞察力.
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