科迪塞宾会影响Streptococcus mutans生物膜,并干扰其新陈代谢
Yidan Shao1, Wenyan Zhu1, Shanshan Liu1,2
1Anhui Key Laboratory of Infection and Immunity, Bengbu Medical University, 2600 Dong Hai Avenue, Bengbu, 233030, China.
BMC oral health
|January 4, 2025
概括
科迪塞宾有效地减少了Streptococcus mutans生物膜生物质和代谢活动,而不会杀死细菌. 这种抗生物膜效应与氨酸和核酸代谢的可逆变化有关.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 药物发现 药物发现 药物发现
背景情况:
- Streptococcus mutans 生物膜具有耐药性,并有助于牙变.
- 了解细菌代谢是开发抗生物膜策略的关键.
研究的目的:
- 为了研究cordycepin对Streptococcus mutans的抗生物膜作用.
- 通过使用代谢学来阐明由cordycepin影响的代谢途径.
主要方法:
- 对浮游生物S. mutans.的最小抑制度 (MIC) 的确定.
- 用cordycepin处理生物膜,然后进行生物质,代谢活性和细胞毒性测试.
- 代谢和定量实时PCR分析以评估代谢变化.
主要成果:
- 科迪塞宾 (128微克/毫升) 降低了S. mutans生物膜生物质和代谢活性,但没有显著的细胞毒性.
- 代谢学揭示了 purin 和核酸代谢的显著变化.
- 观察到的代谢变化在很大程度上在停止治疗后是可逆的.
结论:
- 科尔迪塞表现出对S. mutans的抗生物膜特性.
- 主要机制涉及 purin 和核酸代谢的调节.
- 科尔迪塞显示出作为对S. mutans生物膜的治疗剂的潜力.
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