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Updated: Jun 4, 2025

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使用So-Smart-seqq捕获单个植入前胚胎的完整转录组的协议

Chunyao Wei1, Jeannie T Lee1

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

STAR protocols
|January 5, 2025
PubMed
概括

链优化的Smart-seq (So-Smart-seq) 从有限的样本提供了全面的转录组分析. 这种方法捕获了各种RNA类型,包括非多基和重复RNA,同时保留了链信息.

关键词:
生物信息学是一种生物信息学.发育生物学是发展生物学.遗传学 是一个遗传学.基因组学就是基因组学.分子生物学分子生物学系统生物学 系统生物学

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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