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相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

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相关实验视频

Updated: Jun 4, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

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使用So-Smart-seqq捕获单个植入前胚胎的完整转录组的协议.

Chunyao Wei1, Jeannie T Lee1

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

STAR protocols
|January 5, 2025
PubMed
概括
此摘要是机器生成的。

链优化的Smart-seq (So-Smart-seq) 从有限的样本提供了全面的转录组分析. 这种方法捕获了各种RNA类型,包括非多基和重复RNA,同时保留了链信息.

关键词:
生物信息学是一种生物信息学.发育生物学是发展生物学.遗传学 是一个遗传学.基因组学就是基因组学.分子生物学分子生物学系统生物学 系统生物学

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Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
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Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
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Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 文字转录学 (Transcriptomics) 是一个学科.

背景情况:

  • 低输入样本对全面的RNA分析构成挑战.
  • 现有的方法可能无法捕获RNA分子的全部光谱,或者可能引入偏差.

研究的目的:

  • 为分析单个小鼠植入前胚胎提供了链优化Smart-seq (So-Smart-seq) 的详细协议.
  • 要突出So-Smart-seq在从低输入样本捕获全面转录组的能力.

主要方法:

  • 单个小鼠的植入前胚胎隔离.
  • 使用So-Smart-seq的图书馆准备,包括核糖体cDNA枯竭.
  • 转录组分析的初始数据处理步骤.

主要成果:

  • 因此,Smart-seq有效地捕获了多基和非多基RNA.
  • 该技术包括重复性RNA,不包括丰富的核糖体RNA.
  • 线程信息被保留,5'到3'的覆盖偏差被最小化.

结论:

  • "So-Smart-seq"是一种可靠的方法,用于对低输入样本的综合转录组分析.
  • 该协议可适应其他低输入生物材料和小RNA检测 (<200 nt).