相关实验视频

Updated: Jun 4, 2025

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

在赞比亚的猪群中首次发现并对轮状病毒C的全基因组进行了鉴定和鉴定

Hayato Harima¹, Yongjin Qiu², Michihito Sasaki³

¹Laboratory of Veterinary Public Health, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.

|January 5, 2025

概括

罗塔病毒C (RVC) 感染小猪,导致胃肠炎. 这项研究确定了赞比亚猪的RVC,揭示了两种新型菌株和在样本人群中53.1%的高流行率.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学

背景情况:

罗塔病毒C (RVC) 是新生猪中急性胃肠炎的重要原因.
非洲猪种群中RVC的分布和流行程度在很大程度上是未知的,这阻碍了有效的疾病管理.

研究的目的:

在赞比亚猪中识别和表征罗塔病毒C菌株.
为了确定RVC感染在赞比亚猪种群中的流行率.

主要方法:

甲基因组分析用于在猪便样本中识别RVC.
在已识别的RVC菌株上进行了全基因组测序.
使用RT-qPCR检查较大的猪便样本队列,以检测RVC存在.

主要成果:

两种不同的RVC菌株ZP18-77-c1和ZP18-77-c2从单个样本中被识别并完全测序.
遗传分析显示,已知的猪RVC菌株具有高核酸序列相同性 (87.7-94.5%).
检测到RVC感染的高患病率,53.1% (78/147) 的样本通过RT-qPCR检测呈阳性.

结论:

这项研究报告了非洲RVC菌株的第一个完整基因组序列.
在赞比亚的猪群中存在RVC感染的显著流行率.
这些发现凸显了对非洲RVC流行病学的进一步调查的需要.

关键词:

完整的基因组序列测序转基因组学是指转基因组学.人类遗传学分析猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪罗塔病毒C型病毒

更多相关视频

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Nasal Wipes for Influenza A Virus Detection and Isolation from Swine

Published on: December 4, 2015

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.