了解碳刷上的微生物过程,在无氧消化过程中加速长链脂肪酸的甲生成
Mou Zhang1, Yilian Han1, Yuanyuan Zeng1
1MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science & Engineering, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin 300350, China.
Water research
|January 5, 2025
概括
添加预先与电活性细菌 (EAB) 适应的碳刷,可以在无氧消化 (AD) 中增强长链脂肪酸甲化. 这种方法通过优化微生物通路和增加对脂肪酸的耐受性来改善富含脂质的废水处理.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 脂质是无氧消化 (AD) 的能量丰富的基质,但它们的水解产生抑制性长链脂肪酸 (LCFA).
- 微生物电解细胞 (MEC) 可以增强AD,但电极电路的必要性以改善直接跨物种电子转移 (DIET) 是有争议的.
- 具有较大的表面积的电极可以在没有外部电源的情况下促进DIET.
研究的目的:
- 为了研究碳刷阳极与电活性细菌 (EAB) 预先适应的有效性,在AD期间增强LCFA甲化.
- 为了比较MEC增强的AD系统中连接和不连接电路的性能.
- 阐明涉及LCFA降解的微生物机制和遗传适应.
主要方法:
- 使用有或没有碳刷阳极的无氧消化 (AD) 系统.
- 比较了应用电位的系统与只有固定电极的系统.
- 采用微生物社区分析和遗传分析来识别关键的微生物和代谢途径.
- 量化的甲生产率和LCFA降解效率.
主要成果:
- 用EAB加速LCFA甲化预习的碳刷阳极.
- 应用的电潜导致了快速的甲生产,但由于同类乙生成和电生成,最大速率降低了5-13%.
- 在只使用碳刷的系统中,甲原体在刷上从Methanosarcina转移到Methanothrix.
- 用EAB进行预富,建立了涉及DIET和物种间气转移的复合机制,优化了LCFA的甲化.
- 微生物对高度LCFA的耐受性进行阐明的遗传调节.
结论:
- 固定电极,特别是与EAB预先适应的碳刷,在AD中显著增强LCFA甲化.
- 由特定细菌驱动的涉及DIET和转移的复合微生物机制是优化LCFA降解的关键.
- 这种方法提高了微生物对LCFA压力的耐受性,为处理富含脂质的废水提供了一个有前途的战略.
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