电子烟引起的细胞应激和线粒体功能的变化
Ramamurthy Chitteti1, Juan Pablo Zuniga-Hertz1, Jorge A Masso-Silva2
1VA San Diego Healthcare System, San Diego, CA, USA; Department of Anesthesiology, School of Medicine, University of California San Diego, USA.
Free radical biology & medicine
|January 5, 2025
概括
电子烟 (e-cig) 的使用会改变血液外体和线粒体功能. 电子烟的气溶会伤害细胞,增加压力和炎症风险.
科学领域:
- 生物医学科学 生物医学科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 电子尼古丁输送系统 (电子烟或电子烟) 越来越受欢迎.
- 吸入电子烟气溶可能会导致除了尼古丁成之外的健康问题.
- 电子烟气溶暴露会影响细胞过程并导致慢性疾病.
研究的目的:
- 调查电子烟使用对年轻成年人循环外体样本的影响.
- 评估电子烟使用者细胞应激的标志物.
- 确定电子烟用户的血如何影响人类细胞中的线粒体功能.
主要方法:
- 在电子烟使用者和非使用者中分析了血外体样本和生物标志物 (mtDNA,蛋白碳基,4-HNE).
- 在体外研究使用暴露于电子烟用户血中的内皮细胞 (EA.hy926) 和上皮细胞 (A549).
- 评估线粒体呼吸,代谢压力,线粒体膜潜力 (MMP) 和活性氧物种 (ROS) 水平.
主要成果:
- 电子烟使用者表现出改变的血外体样本,增加了无细胞线粒体DNA (mtDNA),蛋白质碳基和4-HNE.
- 来自电子烟使用者的血减少了暴露细胞中的最大线粒体呼吸和备用容量.
- 在暴露于电子烟用户血的细胞中观察到增加的代谢压力,改变的线粒体形态,升高的MMP和升高的ROS水平.
结论:
- 电子烟的使用导致了血外体样本的显著改变,并增加了线粒体应激的标志物.
- 电子烟使用者血中的循环因子在内皮和上皮细胞中驱动代谢压力.
- 电子烟使用对线粒体功能产生不利影响,可能导致慢性炎症.
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