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模仿瘤微环境的巨细胞纳米纤维作为结直肠癌的向治疗平台

Edoardo D'Angelo¹, Riccardo Rampado², Francesca Sensi³

¹General Surgery 3, Department of Surgery, Oncology and Gastroenterology, University of Padova, via Giustiniani 2, 35128 Padua, Italy; NanoInspired Biomedicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy.

International journal of pharmaceutics

|January 5, 2025

概括

来自抗瘤巨细胞 (M1) 的仿生纳米颗粒显示了对结直肠癌 (CRC) 治疗的增强向性和降低毒性. 这些M1-LNV为精准医学在癌症治疗中提供了一个有希望的新途径.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症生物学癌症生物学

背景情况:

巨细胞在结直肠癌 (CRC) 瘤微环境中发挥着至关重要的作用.
针对性地向癌细胞提供治疗药物是CRC治疗的一个关键挑战.
在纳米粒子设计中利用免疫细胞特性提供了一种新的治疗策略.

研究的目的:

开发和表征生物模拟脂质纳米纤维 (LNVs) 使用分化抗瘤巨细胞 (M1) 的膜蛋白用于CRC向.
为了评估M1衍生LNVs载有多克索鲁比 (DOXO) 的体外和体内疗效和安全性.
探索M1-LNVs作为CRC治疗的精密纳米医学方法的潜力.

主要方法:

从THP-1细胞和M1巨细胞中分离膜蛋白.
通过微流体产生T-LNV和M1-LNV,装载着多克索鲁比 (DOXO).
对LNVs的表征 (大小,PDI,泽塔潜力,药物负载),用于蛋白质确认的质谱学,体外细胞培养研究和体内斑马鱼胚胎模型.

主要成果:

M1-LNVs和T-LNVs表现出类似的物理化学特性和高药物加载效率.
质谱学证实了膜蛋白的存在,T-LNVs和M1-LNVs之间的蛋白质表达有显著差异.
在体外和体内研究表明,M1-LNV与T-LNV相比,M1-LNV的向,吸收和细胞毒性优于癌细胞,系统毒性低于自由DOXO.

结论:

M1-LNVs显示了针对结直肠癌的增强准和治疗疗效.
使用M1巨细胞膜蛋白的仿生方法为基于纳米粒子的癌症治疗提供了一个多功能平台.
在结直肠癌治疗中,M1-LNVs代表了精密纳米医学的一个有前途的战略.

关键词:

生物模拟学是一种生物模拟学.结肠直肠癌是一种癌症.脂质纳米微粒是什么意思瘤向,斑马鱼模型

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