GmRPN11d通过SUMOylation改善蛋白质稳定性,积极调节植物的盐分耐受性
Sibo Wang1, Jingsong Guo1, Yanyan Peng1
1College of Life Science, Northeast Agricultural University, Harbin 150030, China.
International journal of biological macromolecules
|January 5, 2025
概括
豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 26S蛋白质酶调节细胞功能必不可少的蛋白质降解.
- 大豆26S蛋白酶子单元GmRPN11d在盐应激下进行上调.
- 了解GmRPN11d的作用是提高植物抗压能力的关键.
研究的目的:
- 研究大豆盐应激反应中的GmRPN11d的功能和调节.
- 探索GmRPN11d作为提高作物盐分耐受性的目标的潜力.
主要方法:
- 在各种压力条件下的基因表达分析.
- 亚细胞局部化研究 (细胞核和细胞质).
- 在阿拉比多普西斯和大豆毛根中过度表达.
- 对盐耐受机制的生理和分子分析.
- 在体内和体外的相互作用测试用GmMMS21.21进行.
- 的SUMOylation试验.
主要成果:
- 在高盐度和压力下,GmRPN11d的表达增加.
- 过度表达GmRPN11d可以提高模型植物和大豆的盐耐受性.
- GmRPN11d改善了反应性氧物种清理和离子稳态.
- 它激活了ABA信号通路,并显示了对ABA的过敏.
- GmRPN11d与GmMMS21相互作用,导致SUMOylation和增强的稳定性.
结论:
- GmRPN11d在促进植物对盐的耐受性方面发挥着重要作用.
- 由GmMMS21介导的SUMOylation增强了GmRPN11d在盐应力下的稳定性.
- GmRPN11d是基因改造开发耐盐大豆品种的有希望的目标.
相关概念视频
Responses to Salt Stress
13.0K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.0K
RNA Stability
33.2K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.2K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
mRNA Stability and Gene Expression
5.5K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.5K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K


