新型内素LysPFX32的表达和特征作为潜在的生物抗菌剂,用于猪肉保存,用于对抗Pseudomonas fluorescens
Peng Guan1, Zixin Ming1, Xi Liu1
1Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
International journal of biological macromolecules
|January 5, 2025
概括
一种新型的菌体内溶素,LysPFX32,可以有效地对抗Pseudomonas fluorescens (P. fluorescens) 和其生物膜. 这种抗菌剂通过减少细菌污染而不会改变肉质,显示出对食品安全的承诺.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 伪体光体 (P. fluorescens) 是一种通过食物传播的病原体.
- 细菌生物膜在食品保存方面带来了挑战.
- 菌体内素提供潜在的抗微生物解决方案.
研究的目的:
- 为了表征新型菌体内溶酶LysPFX32.2.
- 评估其对P. fluorescens及其生物膜的抗菌活性.
- 评估其在食品保存方面的安全性和有效性.
主要方法:
- LysPFX32.32 的表达和特征.
- 分子对接以预测结合.
- 最低抑制度 (MIC) 的测定.
- 扫描电子显微镜 (SEM) 扫描电子显微镜.
- 生物膜抑制和去除试验.
- 血液溶解和细胞毒性测定.
- 在猪肉中进行食品保存试验.
主要成果:
- LysPFX32 证明了有效的结合到糖.
- 它对P. fluorescens表现出强烈的溶解活性,增加了膜的透性.
- LysPFX32 抑制了生物膜的形成,并消除了成熟的生物膜.
- 恩多利辛显示出极好的生物安全性,没有显著的血液溶解或细胞毒性.
- 莱斯PFX32有效地根除了猪肉中的P.光体,在储存期间保持了肉质.
结论:
- LysPFX32 是一种强大的抗菌剂,可以对抗P. fluorescens及其生物膜.
- 它在食品应用中具有有利的安全性和有效性概况.
- 菌体内素是控制食品中P. fluorescens污染的有希望的策略.
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