来自Teucrium quadrifarium的新型新克莱罗丹二烯酸和它们的抗菌作用
Huan Wang1,2, Han-Fei Liu1,2, Xiao-Qiao Yang1,2
1State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, 550014, People's Republic of China.
Natural products and bioprospecting
|January 5, 2025
概括
在Teucrium quadrifarium中发现了四种新型的新克莱罗丹二甲. 化合物1和12在HT-22细胞中显示出显著的抑制对铁亡的作用,这表明了潜在的治疗应用.
科学领域:
- 自然产品 化学 化学
- 药用化学 医学化学
- 有机化学 有机化学
背景情况:
- 铁四 (Teucrium quadrifarium) 是生物活性化合物的植物来源.
- 新克莱罗丹二甲类代表了一类具有多样化生物活动的天然产品.
- 铁亡是治疗干预的新兴目标.
研究的目的:
- 从Teucrium quadrifarium中分离和表征新的新克莱罗丹二次体.
- 评估这些化合物对HT-22细胞中铁亡的抑制作用.
- 研究这些天然产品作为治疗剂的潜力.
主要方法:
- 使用色谱技术分离和净化化合物.
- 通过光谱方法 (NMR,MS) 和X射线晶体学来阐明新化合物的结构.
- 使用ECD计算确定绝对配置.
- 在实验室中使用RSL3诱导的HT-22细胞中的ferroptosis模型对抗ferroptosis活性的评估.
- 对反应性氧物种 (ROS) 积累的测量.
主要成果:
- 四种新的新克莱罗丹二烯酸,Teucrifarides A-D (1-4),与十六种已知的类似物一起被分离出来.
- 化合物1和12在HT-22细胞中显著抑制RSL3诱导的铁亡,EC50值分别为11.8±1.0μM和4.52±1.24μM.
- 化合物1还诱导治疗细胞中的活性氧物种 (ROS) 积累.
- 对于化合物1和2,发现了新的骨结构.
结论:
- 铁四体是结构独特的新克莱罗丹四体的丰富来源.
- 化合物1和12表现出强烈的抗ferroptotic活性,表明它们作为治疗的潜力.
- 进一步研究作用机制,特别是ROS的作用是有必要的.
关键词:
新克莱罗丹新克莱罗丹泰乌克里姆四方体 (Teucrium quadrifarium) 是一个四方体 (Teucrium quadrifarium).铁代抑制活性 铁代抑制活性兰花科 (Lamiaceae) 是一个植物.这就是ROSOS ROS.更多相关视频
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