1950年SRM的全面,定量分析:NIST的人类等离子体参考材料
Rupasri Mandal1, Jiamin Zheng1, Lun Zhang1
1Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.
Analytical chemistry
|January 6, 2025
概括
这项研究全面分析了NIST人类血SRM 1950,使用多种分析技术量化了1058种代谢物. 这些结果为代谢学校准和比较工作提供了有价值的基准.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 目标代谢学依赖于准确的分析方法来定量代谢物.
- 通过结合多种分析技术,通常可以实现全面的代谢组覆盖.
- 美国国立研究所的人体血参考材料SRM 1950被广泛用于校准和验证.
研究的目的:
- 为了全面和定量地描述NIST的人类血SRM 1950.
- 提供大量,经过充分验证的代谢物度值 (基准) 清单.
- 帮助代谢学界进行校准和比较工作.
主要方法:
- 结合了四个分析平台:高分辨率的NMR光谱学,直接注射并列MS (DI-MS/MS),LC-MS/MS和ICP-MS.
- 使用了八个经过验证的分析试验,以获得准确的定量测量.
- 进行计算机辅助的文献挖掘,以识别先前量化的代谢物.
主要成果:
- 通过使用联合分析平台和测试,定量表征了728种独特的代谢物.
- 通过文献挖掘确定了另外330种独特的代谢物.
- 在SRM 1950中生成了1058种代谢物/代谢物种的高可靠性列表,包括各种化学类.
结论:
- 这项研究提供了迄今为止SRM 1950的最完整的定量表征.
- 生成的数据为代谢学社区提供了必要的基准.
- 在线数据库 (SRM1950-DB) 免费提供详细的代谢物信息.
相关概念视频
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