乳酪衍生抗菌素阳离子与模型膜和细胞的相互作用
Melania M Noe1,2,3, Jésica A Rodríguez4,5, Gabriela R Barredo Vacchelli4,5
1Departamento de Química, Catedra de Química Biológica, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba 5000, Argentina.
Langmuir : the ACS journal of surfaces and colloids
|January 6, 2025
概括
这项研究揭示了抗微生物β-乳糖球蛋白 (β-lg) 125-135如何与模型细胞膜相互作用. 该首选准并改变细菌膜而不是真核细胞膜,这表明其抗微生物作用的机制.
科学领域:
- 生物化学 生物化学
- 膜生物物理学 膜生物物理学
- 抗微生物是一种抗菌.
背景情况:
- 抗微生物对宿主防御至关重要,代表了传统抗生素的有希望的替代品.
- 了解抗微生物与宿主细胞膜相互作用的精确机制对于开发新的治疗剂至关重要.
- β-乳糖球蛋白 (β-lg) 是一种主要的牛奶蛋白,具有潜在的抗菌特性,特别是在水解后.
研究的目的:
- 阐明离子抗微生物β-lg125-135与模型真核生物 (dpPC) 和细菌 (dpPC:dpPG) 膜的相互作用机制.
- 调查的电荷和膜组成如何影响其吸附,透和膜扰动.
- 为了确认该的杀菌活性和对真核细胞的安全性.
主要方法:
- 对暴露于β-lg125-135的脂质单层 (dpPC和dpPC:dpPG) 的表面压力测量和压缩等温度.
- 使用DPH和TMA-DPM探针在dpPC和dpPC:dpPG的小单囊 (SUV) 中进行光异质性 (FA) 测定.
- 在真核细胞细胞系上确认抗微生物活性对抗格拉姆阳性细菌和细胞毒性评估.
主要成果:
- 与zwitterionic dpPC相比,β-lg125-135对负电荷的dpPC:dpPG单层的透和吸附增加.
- 该具有较高的吸附/脱附率和较低的与dpPC:dpPG的关联常数,表明了短暂的相互作用.
- 含有β-lg125-135的单层显示出改变的表面特性,包括压缩性降低和表面潜力增加,特别是在dpPC:dpPG.
- 在SUV中,β-lg125-135破坏了dpPC二层,但增加了dpPC:dpPG二层的异性质,表明膜硬.
结论:
- 由于静电吸引力,β-lg125-135显示出对负电荷 (细菌) 膜接口的强烈偏好.
- 该与细菌膜的相互作用的特点是有利的方向,但稳定性和停留时间较低.
- 该有效地使细菌模型膜变硬,为其拟议的抗菌作用机制做出贡献.
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