Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

3.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.7K
Proteoglycans01:05

Proteoglycans

3.9K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
3.9K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

11.3K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
11.3K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K
Transport Across the Golgi01:26

Transport Across the Golgi

4.1K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
4.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Systemic Type I Interferon Blockade Mitigates Insulin Resistance in a Preclinical Model of Psoriasis.

European journal of immunology·2026
Same author

Copper-Assisted Oxidative One-Pot Heterodifunctionalization of Cyclopentane-1,3-diones.

The Journal of organic chemistry·2026
Same author

Microplastics abundance in major tributaries and distributaries of the river Ganga at lower estuarine stretch.

Marine pollution bulletin·2025
Same author

Oxidative functionalization of cyclopentane-1,3-diones: cell imaging studies of diphenothiazine derivatives.

Organic & biomolecular chemistry·2025
Same author

Integrating machine learning models for optimizing ecosystem health assessments through prediction of nitrate-N concentrations in the lower stretch of Ganga River, India.

Environmental science and pollution research international·2025
Same author

Pediatric to adult primary care transition for medically complex youth: A tale of learning from challenges experienced implementing a pilot project during COVID-19.

Health care transitions·2024
Same journal

Corrigendum to: Reviewing the Context of Molecular Modeling to Enhance the Application of Machine Learning Technologies for Safer Bioinformatics.

Protein and peptide letters·2026
Same journal

Corrigendum to: A Preliminary Study on the Antibacterial Activity of the Secretion of the Levantine Water Frog, <i>Pelophylax bedriagae</i> (Camerano, 1882) (Anura:Ranidae).

Protein and peptide letters·2026
Same journal

Potent Antioxidant and Antibacterial Activities of ≤3 kDa Hydrolyzed Sarcoplasmic Proteins from IPB-D1 Chicken.

Protein and peptide letters·2026
Same journal

Hybrid 3D Bioprinted Scaffolds for the Delivery of Peptide Therapeutics.

Protein and peptide letters·2026
Same journal

Targeting α-Synuclein: Current Strategies and Emerging Therapies for Synucleinopathies.

Protein and peptide letters·2026
Same journal

Biocompatible Excipients from Microalgae: Advancing Protein and Peptide Therapeutics through Sustainable Formulation Strategies.

Protein and peptide letters·2026
查看所有相关文章

相关实验视频

Updated: Jun 3, 2025

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

3.8K

对UDG的结构和功能洞察力

Shreya Roy1, Md Khabeer Azhar1,2, Vibha Gupta1

  • 1Department of Biotechnology, Jaypee Institute of Information Technology, A-10 Sec 62, Noida, 201309, India.

Protein and peptide letters
|January 6, 2025
PubMed
概括
此摘要是机器生成的。

乌拉DNA糖酶 (UDGs) 通过去除乌拉来修复DNA损伤. 来自Mycobacterium smegmatis的一个独特的UDGX形成了一个稳定的DNA复合体,可能保护基因组,结构洞察力有助于神经应用.

关键词:
基础切割维修 基础切割维修这是一个R环.UDGX UDGX 的意思诊断. 诊断. 诊断. 这就是诊断.结构 结构 结构 结构乌拉 DNA 葡萄糖酶酶

更多相关视频

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
13:35

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

173
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.7K

相关实验视频

Last Updated: Jun 3, 2025

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

3.8K
Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota
13:35

Structural Biology and Analytical Chemistry Approaches for Characterizing C-Glycoside Metabolic Enzymes in Human Gut Microbiota

Published on: May 23, 2025

173
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

10.7K

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 遗传学 是一个

背景情况:

  • 在所有生命领域中,DNA损伤需要修复途径.
  • 乌拉DNA糖酶 (UDG) 是基切除修复 (BER) 途径中的关键酶,从DNA中去除乌拉.
  • UDG被分为六个家族,共享DNA结合和催化过程的保存动机.

研究的目的:

  • 审查UDG家族的结构和功能方面.
  • 突出了来自Mycobacterium smegmatis的非传统UDGX的独特特征.
  • 探索UDG的潜在的术语应用.

主要方法:

  • 包括UDGX在内的UDG家族的结构分析.
  • 检查UDG-DNA相互作用和催化机制.
  • 审查现有的文献和PDB结构数据.

主要成果:

  • 不同于其他UDG,UDGX与DNA形成了一个不可逆转的,高度稳定的复合体.
  • 这种UDGX机制防止了 uracil 切除,并可能保护基因组完整性.
  • 低氧下的细菌存活率因MSMUDGX过度表达而减少.
  • 可提供apo和DNA结合的MsmUDGX的高分辨率3D结构.

结论:

  • UDGX代表了一种非传统的UDG,具有独特的DNA结合机制.
  • 自杀性无活化UDGX可能会赋予基因组保护,特别是在压力下.
  • 了解UDG的结构和功能对于开发theranostic工具至关重要.