通过TLR4/MAPK/NF-κB通路调节,素酸对过敏性鼻炎的治疗作用
Xiaoyan Xu1, Lei Wang1, Guangyao Wu1
1Otolaryngology Head and Neck Surgery, China Resources & Wisco General Hospital, Wuhan, Hubei, China.
Biomolecules & biomedicine
|January 6, 2025
概括
酸 (CGA) 通过抑制TLR4/MAPK/NF-κB通路,减少细胞中的炎症,并缓解小鼠过敏性鼻炎 (AR) 症状. 这突出了CGA的重点.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 生物化学 生化学
背景情况:
- 炎症状况和过敏性鼻炎 (AR) 给健康带来了重大挑战.
- 酸 (CGA) 显示出潜在的抗炎性质.
- 了解CGA作用背后的分子机制对于治疗开发至关重要.
研究的目的:
- 为了研究酸 (CGA) 对RAW264.7巨细胞炎症的治疗作用.
- 评估CGA在卵蛋白 (OVA) 诱导过敏性鼻炎 (AR) 的小鼠模型中的疗效.
- 阐明托尔类受体-4 (TLR4) /MAPK/NF-κB信号通路在CGA抗炎作用中的作用.
主要方法:
- 试验室研究涉及治疗具有不同CGA度的脂聚糖 (LPS) 诱导的RAW264.7细胞,Tlr4沉默基因 (shTlr4) 转染以及MAPK/NF-κB通路激活剂.
- 试验包括CCK8用于细胞活力,Griess色度测定用于氧化 (NO),TNF-α的免疫光学,IL-6的ELISA,qPCR,以及用于途径分析的西部涂抹.
- 在体内研究中使用了一种卵泡胺 (OVA) 诱导的AR小鼠模型,用不同的CGA剂量治疗,诱导TLR4过度表达以评估途径调制.
主要成果:
- CGA显著降低了LPS诱导的RAW264.7细胞活性,NO,TNF-α和IL-6分泌.
- CGA下调了Tlr4,p-p38,p-p65和p-IκB的表达,表明抑制了MAPK/NF-κB通路.
- 在OVA诱导的AR小鼠中,CGA有效地减轻了过敏症状,减少了炎症因素,并抑制了TLR4/MAPK/NF-κB通路活性.
结论:
- 酸 (CGA) 在细胞和动物模型中表现出显著的抗炎作用.
- 在炎症状况和过敏性鼻炎 (AR) 中,CGA的治疗潜力通过调节TLR4/MAPK/NF-κB信号通路进行调节.
- 这些发现支持CGA作为一种有前途的治疗剂,用于管理炎症性疾病.
更多相关视频
03:40Acupoint Catgut Embedding Therapy in Traditional Chinese Medicine for Managing Allergic Rhinitis
Published on: December 20, 2024
401
07:36Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
Published on: December 23, 2022
1.5K
相关概念视频
Antiasthma Drugs: Leukotriene Modifiers
241
Leukotriene modifiers, or cysteinyl leukotriene receptor antagonists, are medications used to manage chronic asthma. These agents target specific inflammatory mediators produced during arachidonic acid metabolism, an essential process in generating inflammation in the body.
Leukotriene modifiers work through two distinct mechanisms:
Leukotriene modifiers work through two distinct mechanisms:
241
NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
GPCRs Regulate Adenylyl Cylase Activity
5.3K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.3K
Inflammatory Response
1.8K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.8K
Allergic Drug Reactions
809
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
809
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
236
Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
236
