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使用双向LSTM与BERT嵌入的双向LSTM预测CRISPR-Cas9在人类初级细胞中的脱效应.

Orhan Sari1, Ziying Liu2, Youlian Pan2

  • 1Department of Mining and Materials Engineering, McGill University, Montreal, QC, H3A 2B1, Canada.

Bioinformatics advances
|January 6, 2025
PubMed
概括

一种新的深度学习模型CrisprBERT准确地预测了集群定期间隔的短时间Palindromic重复 (CRISPR) -Cas9的目标外效应. 该工具通过先进的序列分析优化单导 RNA 设计,提高基因组编辑效率.

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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...