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评估饮用水中的微生物生长,使用核酸含量和流式细胞计指纹

Leila Claveau¹, Neil Hudson², Paul Jeffrey¹

¹Cranfield University, College Road, Cranfield, Bedfordshire MK43 0AL, UK.

|January 6, 2025

概括

流细胞计揭示了高和低核酸细菌如何响应饮用水处理的变化. 这种方法提供了一种更敏感的方式来监测水质和细菌动态.

科学领域:

环境微生物学环境微生物学
水质监测水质监测
细菌生理学细菌生理学

背景情况:

饮用水系统中的细菌种群表现出异质性.
了解细菌动态对于确保水的安全性和处理效率至关重要.
传统的监测方法可能无法完全捕捉微生物群落的复杂性.

研究的目的:

评估使用流式细胞计 (FCM) 来根据核酸含量区分细菌群.
评估水处理工艺 (,营养物质) 对细菌动态的影响.
探索FCM指纹与集群分析,以加强水质评估.

主要方法:

使用流细胞计 (FCM) 来分析完整的细菌细胞.
将种群分为高核酸 (HNA) 和低核酸 (LNA) 含量.
将集群分析应用于FCM数据,以进行全面的微生物分析.

主要成果:

和营养水平不同地影响HNA和LNA细菌种群.
HNA 细菌在对营养变化的反应中显示出快速生长.
LNA细菌反映了适应稳定,低有机环境的情况.
水处理条件显著改变HNA/LNA比率.

结论:

FCM是监测饮用水中的细菌动态的一个有价值的工具.
HNA/LNA细菌作为特定生理状态和对治疗的反应的指标.
FCM指纹与集群分析相结合,为水质评估提供了更高的灵敏度.
建议对先进的水处理和供应监测进行多参数数据分析.

关键词:

环境化学环境化学环境健康环境健康环境科学环境科学

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Flow Cytometry

Flow Cytometry

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