模拟两个Listeria单细胞菌株的生长,持久性和非持久性:温度的影响
Ľubomír Valík1, Jana Minarovičová2, Eva Kaclíková2
1Department of Nutrition and Food Quality Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia.
Heliyon
|January 6, 2025
概括
与非持久菌株相比,持久性Listeria monocytogenes菌株在高温 (40-43°C) 时的生长率显著更高. 这种生长动力学的差异,特别是在最佳温度以上,可能会导致Listeria monocytogenes在食品加工环境中的持久性.
科学领域:
- 食品微生物学 食品微生物学
- 细菌生长动力学 细菌生长动力学
- 食品安全 食品安全
背景情况:
- 在食品加工环境中细菌的持久性是一个重要的食品安全问题.
- 增强的生长特征可以促进Listeria monocytogenes的持久性.
- 了解影响Listeria monocytogenes生长的因素对于制定有效的控制策略至关重要.
研究的目的:
- 研究温度对持久性 (LM-P) 和非持久性 (LM-S) Listeria monocytogenes菌株的浮游生物生长的影响.
- 用巴兰尼和枢纽温度模型量化描述生长参数.
- 确定生长速度的差异,特别是在更高的温度下,是否与Listeria monocytogenes的持久性相关.
主要方法:
- 在Tryptone豆 (TSB) 和半合成奶酪介质 (SCM) 中培养持久性 (LM-P) 和非持久性 (LM-S) 的Listeria monocytogenes菌株.
- 在6°C至43°C的温度范围内进行化.
- 在增长数据分析中应用巴拉尼和枢纽温度模型.
主要成果:
- 从6°C到37°C的菌株之间没有观察到显著的生长速度差异.
- 显著更高的平均增长率 (p <0.05) 发现LM-P相比LM-S在40°C和43°C在两个介质.
- 核心温度模型显示出不同的生长参数,LM-P通常表现出高于最佳温度的较高生长率.
结论:
- 增长速度的差异,特别是在高于最佳温度的温度下,可能在Listeria monocytogenes的持久性中发挥作用.
- 该研究提供了定量增长数据,可以为Listeria monocytogenes在食品环境中的行为提供预测模型的信息.
- 这些发现表明,高温可能是某些Listeria monocytogenes菌株持续存在的关键因素.
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