相关实验视频

Updated: May 7, 2025

Author Spotlight: Evaluating Traditional Chinese Therapy for Ankylosing Spondylitis in Mice

Author Spotlight: Evaluating Traditional Chinese Therapy for Ankylosing Spondylitis in Mice

Published on: October 27, 2023

在结性脊髓炎中探索分子生物标志物转录学

Yuanpiao Ni^1,2, Linrui Zhong^1,2, Yanhui Li^1,2

¹Research Center of Hyperuricemia and Gout, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China.

Frontiers in immunology

|January 6, 2025

概括

这项研究确定了五个关键基因 (ACSL1,SLC40A1,GZMM,TRIB1,XBP1) 作为诊断结性脊髓炎 (AS) 的潜在生物标志物. 这些基因具有很高的诊断准确性,与疾病活动相关,提供新的治疗点.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

化脊柱炎 (AS) 是一种慢性,渐进的炎症性疾病,影响脊柱和关节.
由于潜伏发作和非特异性症状导致AS的延迟诊断可能导致残疾.
识别新的生物标志物对于早期诊断和AS的有效管理至关重要.

研究的目的:

通过生物信息学分析,识别与结性脊柱炎 (AS) 相关的关键基因.
评估已识别的基因作为AS潜在生物标志物的诊断准确性.
探索这些基因,免疫细胞透和AS的临床指标之间的相关性.

主要方法:

对GEO数据集进行了差异基因表达分析和加权基因共表达网络分析 (WGCNA).
拉索回归确定了关键基因,ROC曲线分析评估了它们的诊断性能.
免疫透分析和RT-qPCR验证了PBMC中的基因表达及其与临床标志物的相关性.

主要成果:

鉴定了六个关键基因;五个 (ACSL1,SLC40A1,GZMM,TRIB1,XBP1) 显示了诊断值 (AUC>0.7).
AS表现出改变的免疫细胞透,以及与疾病活性相关的基因表达.
通过RT-qPCR确认了ACSL1,SLC40A1,GZMM和XBP1的表达模式,TRIB1显示了反向相关性.

结论:

已识别的基因可能与铁亡有关,作为AS的有前途的诊断生物标志物.
周围血液单核细胞 (PBMC) 中的基因表达与AS疾病活性相关.
这些发现为开发新的诊断工具和AS治疗策略提供了有价值的见解.

关键词:

结性脊柱炎是一种疾病.不同的基因表达方式铁性化 (ferroptosis) 是一种免疫透分析免疫透分析转录组学转录组学是指转录组学.权重基因共同表达网络分析.

更多相关视频

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...