验证高性能液态染色法,用于在L. Cultivar Dangjojo中确定Quercitrin
Ardina Nur Fauziah1, Min Jeong Kim1, Bo Ram So1
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea.
Preventive nutrition and food science
|January 6, 2025
概括
一种新的HPLC方法准确地量化了Dangjo (DJ) 提取物中的Quercitrin. 这种经过验证的分析方法确保了DJ的可靠质量控制,这是一种具有潜在健康益处的新作物.
科学领域:
- 农业科学 农业科学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- Capsicum annuum L. cultivar Dangjo (DJ) 是一种新型作物,具有潜在的健康益处,包括降低血糖.
- DJ含有黄类糖化物,如酸丁,这是具有药理作用的生物活性化合物.
- 在DJ中标准化化素量化对于其在食品行业的采用至关重要.
研究的目的:
- 开发和验证一种高性能液态染色学 (HPLC) 方法,用于在DJ提取物 (DJEs) 中准确量化氏素.
- 确保质量控制和进一步研究的分析方法的可靠性和准确性.
主要方法:
- 在DJE样本中开发HPLC检测Quercitrin的方法.
- 对HPLC方法的验证,包括对线性,准确性 (回收研究) 和精度 (可重复性和可重复性) 的评估.
- 结果与标准西溶液的比较.
主要成果:
- 通过HPLC方法在DJE样本中有效检测到素,与标准相比显示一致的保留时间和峰值光谱.
- 在2.515.0微克/毫升 (R2 > 0.9997) 的度范围内,线性得到证实.
- 精度 (89.02%99.30%回收) 和精度 (RSD ≤5.95%) 在可以接受的范围内,符合AOAC标准.
结论:
- 已经建立了一个验证的HPLC方法,用于在DJE中定量化Quercitrin.
- 这种方法促进了DJ的标准化,并确保了质量控制的准确分析.
- 经过验证的方法支持进一步研究DJ的促进健康的特性.
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