相关实验视频

Updated: Jun 3, 2025

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis

Published on: February 8, 2017

氨酸5AC 通过cMET/CD44v6促进乳腺癌大脑转移

Shailendra Kumar Maurya¹, Jenny A Jaramillo-Gómez², Asad Ur Rehman¹

¹Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska.

Clinical cancer research : an official journal of the American Association for Cancer Research

|January 6, 2025

概括

乳腺癌大脑转移时,素5AC (MUC5AC) 被上调调节,并通过cMET/CD44v6途径驱动瘤的扩散. 用bozitinib准这个轴为这种情况提供了一种新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症转移研究癌症转移研究

背景情况:

乳腺癌大脑转移是一个重大的临床挑战.
在这个过程中,粘蛋白,特别是粘蛋白5AC (MUC5AC) 的作用尚不清楚.

研究的目的:

研究MUC5AC在乳腺癌大脑转移中的作用.
在MUC5AC路径中识别潜在的治疗点.

主要方法:

在患者数据的形转录学分析.
患者样本和细胞系的RNA序列分析.
在体外和体外功能测定,包括细胞迁移和粘附研究.
共同免疫沉以确定蛋白质相互作用.
在小鼠模型中使用cMET抑制剂bozitinib进行治疗.

主要成果:

在乳腺癌脑转移中,MUC5AC的调节显著上升,并且与HER2+亚型的生存率差相关.
在患者血清中检测到MUC5AC水平升高.
在体内,MUC5AC沉默减少了细胞迁移和大脑转移.
MUC5AC通过cMET/CD44v6轴促进转移,通过肝细胞生长因子信号激活.
博西提尼布通过向这一轴,有效地抑制乳腺癌大脑转移.

结论:

MUC5AC/cMET/CD44v6轴对乳腺癌大脑转移至关重要.
准这一轴代表了管理乳腺癌大脑转移的新治疗策略.

更多相关视频

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Published on: February 4, 2021

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

Published on: December 26, 2016

相关概念视频

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...