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PRDM9驱动了鱼类鱼类重组热点的位置和快速演变

Marie Raynaud¹, Paola Sanna², Julien Joseph³

¹ISEM, Univ Montpellier, CNRS, IRD, Montpellier, France.

|January 6, 2025

概括

PRDM9基因驱动了鱼类的重组热点的快速演变,类似于哺乳动物. 这表明PRDM9是PRDM9.

科学领域:

遗传学遗传学是一个
进化生物学进化生物学
分子生物学分子生物学

背景情况:

介质重组发生在真核生物中的特定热点.
在哺乳动物中,PRDM9驱动了从促销者那里的重组,导致了快速的热点进化.
在一些血统中PRDM9的祖先作用和损失引发了关于其保存功能的问题.

研究的目的:

研究沙门类动物中PRDM9的功能,以了解其在化过程中的保留作用.
分析重组景观和PRDM9对鱼类物种热点的影响.

主要方法:

绘制了介质DNA双链断裂 (DSB) 的地图,以确定Oncorhynchus mykiss.中的重组启动地点.
分析了PRDM9基因型依赖的热点局部化.
检查了PRDM9指域多态性.
生成了O. mykiss,Oncorhynchus kisutch和Salmo salar. 的种群规模重组地图.

主要成果:

在远离发起体的热点聚集的DSB,富含H3K4me3和H3K36me3,并依赖PRDM9基因型.
高PRDM9指域多态性表明积极选择.
由于PRDM9标动机侵蚀,重组热点的快速转换在鱼类中被观察到.

结论:

在脊椎动物中,PRDM9在调节重组热点方面的功能是保留的.
由PRDM9驱动的快速进化动态已经活跃了数百万年.

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