Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Assessing the limitations of paraformaldehyde fixation for accurate cell surface receptor measurement.

Frontiers in pharmacology·2026
Same author

Short- and Long-Term Effects of Sodium Phenylbutyrate on White Matter and Sensorimotor and Cognitive Behavior in a Mild Murine Model of Encephalopathy of Prematurity.

International journal of molecular sciences·2025
Same author

Natural L-Hexapeptides with Therapeutic Potential for Alzheimer's Disease and Tauopathies.

ACS omega·2025
Same author

Microalgae empower skeletal muscle via increased force production and viability.

Science advances·2025
Same author

A coiled-coil domain triggers oligomerization of MmpL10, the mycobacterial transporter of trehalose polyphleate precursor.

FEBS letters·2025
Same author

Molecular Assemblies of Amphiphilic Oligothiophenes at the Air-Water Interface.

Langmuir : the ACS journal of surfaces and colloids·2025

相关实验视频

Updated: Jun 3, 2025

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
06:34

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Published on: March 27, 2017

11.9K

有效的生化方法来表征和分类相关的氨基原蛋白.

Lucas Pradeau-Phélut1, Stacy Alvès2, Léo Le Tareau1

  • 1Institut de Recherche en Santé, Environnement et Travail (Irset)─Inserm─EHESP, UMR_S 1085, Université de Rennes, 9 av. du Professeur Léon Bernard, F-35042 Rennes, France.

Analytical chemistry
|January 6, 2025
PubMed
概括

研究人员开发了一种新的生化方法来分类涉及蛋白质病变的氨基基. 这种方法有助于理解蛋白纤维核和开发针对性治疗amyloidosis.

更多相关视频

Rapid Generation of Amyloid from Native Proteins In vitro
05:48

Rapid Generation of Amyloid from Native Proteins In vitro

Published on: December 5, 2013

6.2K
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.0K

相关实验视频

Last Updated: Jun 3, 2025

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
06:34

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Published on: March 27, 2017

11.9K
Rapid Generation of Amyloid from Native Proteins In vitro
05:48

Rapid Generation of Amyloid from Native Proteins In vitro

Published on: December 5, 2013

6.2K
Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain
09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

Published on: April 28, 2022

3.0K

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 蛋白质科学 蛋白质科学

背景情况:

  • 氨基粉症涉及蛋白质沉积形成氨基粉纤维,影响各种器官.
  • 了解蛋白质核和延长对于开发有效疗法至关重要.
  • 小 (5-10个氨基酸) 是粉样纤维核化的关键.

研究的目的:

  • 开发一种新的生物化学方法来表征和分类氨基类.
  • 为了克服在核化过程中涉及的小的in silico方法的局限性.
  • 为了更好地了解粉样蛋白形成中的分子事件.

主要方法:

  • 开发了一种新的生物化学方法,用于的表征和分类.
  • 使用加尼耶-德拉马尔什图表进行分类.
  • 在微管相关蛋白 (MAPT) 的PHF6 (β1) 和22种相关上测试了该方法.

主要成果:

  • 酸被分为三个组:聚合性,粉样性和可溶性.
  • 使用PHF6和相关来证明该方法的有效性.
  • 展示了Garnier-Delamarche图表在类分类中的适用性.

结论:

  • 这种新型的生物化学方法准确地描述和分类氨基类.
  • 这种分类有助于理解粉样核和开发疗法.
  • 该方法广泛适用于各种类家族,并且可用于高通量分析.