相关实验视频

Updated: May 7, 2025

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

在Lilium pumilum中,NAC转录因子LpNAC48促进了三体的形成

Yin Xin^1,2, Wenqiang Pan^1,2,3, Yajie Zhao²

¹Institute of Grassland, Flowers, and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Plant physiology

|January 6, 2025

概括

在Lilium pumilum中,三角体的发育受到NAC和BBX转录因子的调节,影响植物应激适应. 通过bZIPTF结合的NAC基因促进体中的DNA变异进一步控制了三体的形成.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
植物压力生理学植物压力生理学

背景情况:

三合体对于植物对环境压力的防御至关重要.
三体形态和密度在不同植物物种之间有很大差异.
了解三合体发育机制是提高作物弹性的关键.

研究的目的:

为了阐明调节三胞体发育的分子机制在Lilium pumilum.
确定关键的转录因子及其与三组形成有关的相互作用.
研究基因变异在三体特征调节中的作用.

主要方法:

基因沉默 (RNAi) 用于评估LpNAC48和LpBBX28.28.的功能.
酵母的一混合和电泳性移动转移试验以确认TF-促进体相互作用.
对基因表达和促进子区域的分析,包括DNA变异识别.

主要成果:

在L. pumilum中,LpNAC48和LpBBX28对于正常的三发育和密度至关重要.
这些TF直接激活bHLH基因LpGL3L的表达.
通过LpbZIP29结合的LpNAC48促进体中的特定DNA变异对其表达和三组形成至关重要.

结论:

一个涉及LpNAC48,LpBBX28和LpbZIP29的监管网络控制了L. pumilum三体的发展.
一个促进者DNA变异在调节基因表达和促进三体特征方面发挥着重要作用.
这项研究为植物三胞体形成和应激适应的遗传基础提供了新的见解.

关键词:

在 LpBBX2828 中,LpGL3L LpGL3L 在线播放在 LpNAC4848 中,这就是LpbZIP29的原因.自然变化的自然变化.百合花的百合花是什么百合花三合体的三合体

更多相关视频

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

相关概念视频

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...