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来自Torreya grandis树皮的diterpenoids及其细胞毒性活动

Yu-Fan Meng¹, Jing-Jing Yuan², Mao-Sheng Zhang³

¹Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi 563000, China; Guilin Hospital of the Second Xiangya Hospital CSU, Guilin 541199, China.

|January 6, 2025

概括

来自Torreya grandis的四种新型双体显示出细胞毒性作用. 化合物6通过JAK2/STAT3通路停止G2阶段进展,显著抑制了HepG2癌细胞.

科学领域:

自然产品化学化学
药理学药理学是指药理学的学科.
细胞生物学细胞生物学

背景情况:

托雷亚大树皮是生物活性天然产品的来源.
迪特尔类是一种具有多种生物活性的化合物.
癌症细胞系研究对于识别潜在治疗剂至关重要.

研究的目的:

从Torreya grandis树皮中分离和表征新型的四面体.
评估这些化合物对人类癌症细胞系的细胞毒性潜力.
为了研究最强效的化合物的作用机制.

主要方法:

使用NMR,HR-ESIMS和ECD计算的化合物的隔离和结构阐明.
通过MTT测定对HCT-116,AsPC-1和HepG2细胞系进行细胞毒性评估.
细胞循环分析和西部抹杀,以探索JAK2/STAT3信号通路.

主要成果:

确定了四种新的二类 (2-5) 和十种已知的类似物.
化合物6对HepG2细胞表现出显著的细胞毒性.
化合物6诱导HepG2细胞的G2阶段停止,与JAK2/STAT3抑制有关.

结论:

托雷亚格兰迪斯树皮产生了具有细胞毒性质的新型二甲.
化合物6是进一步研究肝癌治疗的有希望的候选者.
JAK2/STAT3通路与化合物6.6的抗癌作用有关.

关键词:

细胞毒性细胞毒性这是一种二甲化物 (Diterpenoid).这是一种HepG2型肝炎.塔克萨属植物 (Taxaceae) 是一个属植物.托雷亚·格兰迪斯 (英语:Torreya grandis) 是一个城市.

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