一个巨型菌体PG216的特征和基因组分析,该菌体对几种Vibrio物种具有广泛的光学活性
Shenao Li1, Xixi Li1, Chen Zhang1
1Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing, Jiangsu, China.
Archives of virology
|January 6, 2025
概括
一种新型的Lytic菌体PG216有效地向多种Vibrio物种,包括*Vibrio parahaemolyticus*. 这种菌体显示出菌体治疗菌体病的潜力,因为它具有广泛的光学活性和缺乏有害基因.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 细菌学 细菌学是一门学科.
背景情况:
- 病是由*Vibrio*物种引起的,对海洋生态系统和人类健康构成重大威胁.
- 抗生素耐药性*Vibrio*菌株的出现需要替代治疗策略.
- 菌体为控制细菌感染提供了一个有希望的替代方案.
研究的目的:
- 为了隔离和表征一种针对*Vibrio parahaemolyticus*的新型菌体.
- 为了评估与各种*Vibrio*物种对抗的隔离菌体的性活性光谱.
- 评估菌体在治疗应用中的潜力,包括生物膜根除.
主要方法:
- 用*Vibrio parahaemolyticus* G299作为宿主从青岛海水中分离出菌PG216.
- 传输电子显微镜用于菌体形态的确定.
- 用于宿主范围的确定,涂层测试的点位和效率.
- 复制参数的一步增长曲线分析.
- 基因组测序和遗传学分析.
- 生物膜根除试验.生物膜根除试验.
主要成果:
- 菌体PG216的头部是二面体 (100nm) 和尾部是收缩型的 (126nm).
- PG216对*V. alginolyticus*, *V. parahaemolyticus*, *V. vulnificus*, *V. mimicus* 和 *V. harveyi* 具有广泛的催化活性.
- 菌体的潜伏期为25分钟,溶解持续时间为115分钟,爆发大小为135个PFU/细胞.
- 基因组分析显示了244,027bp的基因组,其中GC含量为42.89%,383个ORF和28个tRNA.
- 遗传学分析将PG216置于类型 * Schizotequatrovirus * 中.
- PG216有效地根除了成熟的 *V. parahaemolyticus* 生物膜.
- 菌体基因组缺乏毒性和抗生素耐药性基因.
结论:
- 菌体PG216是一种新型的菌体,在*Vibrio*属内具有广泛的宿主范围.
- PG216显示出高效的复制和生物膜消除能力.
- 毒性和抗生素耐药性基因的缺失使PG216成为菌体治疗vibriosis的有希望的候选人.
相关概念视频
01:30Lytic Cycle of Bacteriophages
70.3K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.3K
00:43Lysogenic Cycle of Bacteriophages
61.8K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
61.8K
01:33Bacterial Transformation
55.1K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
55.1K
00:46Genomic DNA in Prokaryotes
43.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.4K