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在活体中,人类神经元交换成像 (NEXI) 在500mT/m的扩散梯度下进行

Kwok-Shing Chan^1,2, Yixin Ma^1,2, Hansol Lee^1,2

¹Athinoula A. Martinos Center for Biomedical Imaging, Charlestown, MA, United States.

bioRxiv : the preprint server for biology

|January 7, 2025

概括

这项研究使用Connectome 2.0扫描仪的先进扩散MRI进行了脑灰质水交换的体内成像. 观察到更快的交换时间,突出了扩散时间和降噪对于精确的神经成像分析的重要性.

科学领域:

神经成像是一种神经成像.
生物物理学的生物物理.
医学物理医学物理

背景情况:

扩散水交换成像 (dEXI) 对于评估大脑微观结构和膜完整性至关重要.
在dEXI的挑战包括实现高的信号噪声比率和短的扩散时间快速交换过程.
精确测量神经元内和细胞外的水交换对于理解神经元功能和疾病至关重要.

研究的目的:

为了证明组织微几何学和人类大脑灰质中的水交换体内成像的可行性.
使用最先进的Connectome 2.0扫描仪与超高性能梯度系统进行先进的扩散MRI.
在灰色物质中应用异型卡尔格模型来估计水交换时间.

主要方法:

在15名健康志愿者身上,使用Connectome 2.0扫描仪进行了扩散MRI测量.
使用多个扩散时间 (13-30毫秒) 和b值高达17.5毫秒/μm2.
适应异型卡尔格模型来估计非交换和交换水群体的停留时间,并使用GPU加速进行分析.

主要成果:

与Connectome 1.0类似系统相比,Connectome 2.0的灰色物质估计的水交换时间明显更快 (中位数为13±8毫秒).
与Connectome 1.0兼容的协议显示,在快速交换过程中,对噪声底部偏差的敏感性增加.
在皮层区域观察到交换时间的空间变化,运动,体感和视觉皮层显示更长的交换时间.

结论:

先进的扩散MRI硬件和优化的协议对于准确的体内测量大脑中的快速水交换是必不可少的.
仔细考虑扩散时间和降噪策略 (例如,解决Rician噪声) 对于可靠的NEXI (神经元交换成像) 估计至关重要.
了解皮层水交换的空间变化可能会提供对区域功能差异和病理学的见解.

关键词:

扩散扩散是一种扩散.交易所交易所交易所灰色物质是一种灰色物质.膜的完整性膜的完整性微观结构的微观结构

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