在S.中映射适应性综合应激反应的遗传结构. 类植物
Rachel Baum1, Jinyoung Kim1, Ryan Y Muller1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
bioRxiv : the preprint server for biology
|January 7, 2025
概括
遗传变化改变了酵母菌的综合应激反应 (ISR) 动态. 翻译受损促进ISR激活,而减少的核糖体容量削弱了它,揭示了蛋白质稳定的机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 综合应激反应 (ISR) 是在压力下维持细胞平衡 (蛋白质稳定) 的关键途径.
- 适当调节ISR强度和速度对于平衡蛋白质合成和细胞健康至关重要.
研究的目的:
- 为了研究遗传干扰如何影响ISR在芽酵母中的动态行为.
- 了解遗传变化,压力水平和ISR活动动态之间的关系.
主要方法:
- 利用CRISPR干扰与条码表达式记者测序 (CiBER-seq) 在数千个遗传扰乱中对ISR动态进行高通量分析.
- 应用硫甲基,通过抑制分支氨基酸合成来诱导可定位应激.
- 测量了随着时间的推移对基因修饰和压力的反应中的ISR记者表达.
主要成果:
- 翻译中断,例如减少氨基酸-tRNA合成酶或tRNA生物发生因子,导致细胞生长减少和ISR激活的比例增加.
- 损伤的核糖体生物生成减少了基础ISR活性和减弱的ISR动态.
- 这些发现表明,减少核糖体容量可能会降低氨基酸需求,影响ISR信号传递.
结论:
- CiBER-seq是测量复杂,动态细胞表型的强大工具.
- 遗传干扰显著影响ISR动态,提供了关于细胞适应和恒温的见解.
- 该研究阐明了翻译和核糖体容量如何调节ISR,从而有助于维持蛋白质稳定.
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