Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K
Ribosome Profiling02:24

Ribosome Profiling

3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The DBD-α4 helix of EWSR1::FLI1 is required for GGAA microsatellite binding that underlies genome regulation in Ewing sarcoma.

eLife·2026
Same author

HES3-dependent regulatory functions in development and fusion-positive rhabdomyosarcoma.

bioRxiv : the preprint server for biology·2026
Same author

Biological Advances and Current Challenges for Pediatric Rhabdomyosarcoma.

Cancers·2026
Same author

Comparative modes of chromatin engagement by PAX::FOXO1 fusions in rhabdomyosarcoma.

bioRxiv : the preprint server for biology·2026
Same author

LSD1 Performs Demethylase-Independent and Context-Specific Roles in Ewing Sarcoma.

Cancer research communications·2026
Same author

LSD1 Performs Demethylase-Independent and Context-Specific Roles in Ewing Sarcoma.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: May 7, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K

RNA-SeqEZPZ:一个点击式管道,用于使用交互式可视化进行全面的转录学分析.

Cenny Taslim, Yuan Zhang, Genevieve C Kendall

    bioRxiv : the preprint server for biology
    |January 7, 2025
    PubMed
    概括

    RNA-SeqEZPZ是一个新的自动化管道,为研究人员简化RNA测序 (RNA-Seq) 分析. 它提供了一个用户友好的界面和命令行选项,用于可复制和高效的基因组数据分析.

    科学领域:

    • 基因组学就是基因组学.
    • 生物信息学是一种生物信息学.
    • 计算生物学 计算生物学

    背景情况:

    • RNA测序 (RNA-Seq) 分析在基因组研究中至关重要,但通常耗时且难以标准化.
    • 许多实验室使用内部脚本,导致可重现性问题和数据分析瓶.
    • 随着RNA测序实验成本的下降,大量数据产生,需要高效的分析管道.

    研究的目的:

    • 开发一个自动化,用户友好的管道,用于严格和可重复的RNA-Seq分析.
    • 提供一种解决方案,消除了对常规分析的编程或生物信息学专业知识的需求.
    • 通过命令行执行和定制,为高级用户提供灵活性.

    主要方法:

    • 开发了RNA-SeqEZPZ,这是一个自动化管道,具有点击界面和命令行选项.
    • 使用bash/SLURM和Nextflow实现了管道,以实现广泛的兼容性.
    • 综合质量控制,对齐,过,读数计数,微分表达和路径分析.
    • 包括一个交互式R闪亮可视化工具,并将所有工具包装在一个奇点图像中,以方便安装和可复制性.
    • 包含批量调整,以尽量减少技术变化影响.

    主要成果:

    • RNA-SeqEZPZ能够从原始读取到功能解释进行全面的RNA-Seq分析.

    更多相关视频

    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
    05:12

    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

    Published on: February 2, 2024

    630
    Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
    10:41

    Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

    Published on: May 9, 2017

    9.2K

    相关实验视频

    Last Updated: May 7, 2025

    Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
    11:52

    Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

    Published on: August 4, 2016

    10.3K
    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
    05:12

    Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

    Published on: February 2, 2024

    630
    Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
    10:41

    Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

    Published on: May 9, 2017

    9.2K
  • 管道通过标准化步骤和容器化 (奇点) 确保可重复性.
  • 交互式可视化方便在实验条件下对差异表达的基因和途径进行比较.

    • 结论:

    • RNA-SeqEZPZ显著减少了RNA-Seq数据分析所需的时间和专业知识.
    • 该管道增强了基因组研究中的标准化和可重复性.
    • 它使研究人员能够高效有效地进行复杂的RNA-Seq分析.