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玉米杂交显示了diel转录模式的等位基因变异

Joseph L Gage^1,2, M Cinta Romay³, Edward S Buckler^3,4,5

¹Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC 27695.

bioRxiv : the preprint server for biology

|January 7, 2025

概括

玉米的基因表达表现出每日节奏,在近亲血统之间自然存在差异. 促进者DNA变异影响这些昼夜节律,影响植物适应不同环境.

科学领域:

植物生物学植物生物学
遗传学遗传学是一个
时间生物学时间生物学

背景情况:

由于昼夜节律和环境线索,基因转录的丰度每天都在波动.
了解这些diel (日常) 转录模式中的等位基因变异以及调节序列的作用是有限的.
玉米 (Zea mays) 是一个具有多样性遗传资源的全球重要作物.

研究的目的:

调查Diel转录丰富度模式的自然变化,跨越多样化的玉米杂交线.
探索cis-regulatory (促进子) 序列对 diel转录模式的影响.
为了确定昼夜基因表达变异,光周期敏感性和玉米的局部适应之间的潜在联系.

主要方法:

在24个不同的玉米杂交系中,量化了diele转录的丰富性.
采用卷积神经网络来预测从促进子序列的基因振荡.
分析了迪尔转录模式与促进物序列哈普洛类型的共分离.

主要成果:

观察到迪尔转录模式的显著自然变异,跨线基因振荡的差异高达两倍.
预测模型确定了与振荡相关的促进器序列中的已知昼夜钟基因结合动机.
具有与促进器单元型相关的 diel 模式的基因被丰富为与光周期敏感性相关的功能.

结论:

调节性基因序列变异显著影响玉米中的基因表达模式.
昼夜转录的变化可能有助于表型可塑性和局部适应,特别是在对日长变化的反应中.
这项研究强调了监管变化的重要性,在作物化和育种期间塑造适应性特征.

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