皮层投射神经元具有明显的轴突连接性,使用具有明显蛋白质组成的核糖体复合体
Tien Phuoc Tran1, Bogdan Budnik2, John E Froberg1
1Department of Stem Cell and Regenerative Biology, and Center for Brain Science, Harvard University, Cambridge, MA, USA.
bioRxiv : the preprint server for biology
|January 7, 2025
概括
在不同的皮层神经元亚型中的核糖体复合体显示出独特的蛋白质组成. 这一发现揭示了在开发过程中指导子类型特定电路形成的翻译控制机制的新见解.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 皮层投射神经元 (PN) 形成各种远程电路,对大脑功能至关重要.
- 翻译控制对于PN发展至关重要,但其在子类型特定电路形成中的作用尚不清楚.
- 核糖体复合体 (RCs) 在蛋白质组成上可以有所不同,从而实现了专门的转化调节.
研究的目的:
- 在不同的皮层投射神经元亚型中研究RCs的亚型特定蛋白质组成.
- 了解这些变化如何促进特定远程轴突电路的形成.
- 为了确定参与神经电路发育过程中翻译调节的新型蛋白质.
主要方法:
- 逆行标签用于识别特定亚型的神经元体.
- 光激活细胞分类 (FACS) 用于细胞净化.
- 核糖体免疫沉和超低输入质谱法用于分析RC蛋白质组成.
主要成果:
- 在从脑下PN (SCPN) 和脑下PN (CPN) 亚型的RC中确定了不同的蛋白质组成.
- 在SCPN RCs中检测到16种关联蛋白质.
- 蛋白激酶Cepsilon (PRKCE) 已被验证为SCPN中的核糖体相关蛋白,具有丰富的基因表达.
结论:
- 核糖体复合体在发育中的皮层投射神经元中表现出亚型特定的蛋白质组成.
- 这些发现突出了包括PRKCE在内的已识别的蛋白质在通过转化控制调节亚型特定电路形成中的潜在作用.
- 这项研究为进一步研究神经发育中RC异质性的功能意义提供了基础.
相关概念视频
Assembly of Complex Microtubule Structures
1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Neurons: The Cell Body and the Dendrites
2.6K
A typical nerve cell comprises three main components: the cell body, dendrites, and the axon. The cell body, also known as the soma or perikaryon, serves as the central biosynthetic hub housing a nucleus surrounded by cytoplasm containing organelles commonly found in most cells. Notably, Nissl bodies, clusters of the rough endoplasmic reticulum and free ribosomes responsible for protein synthesis, are distinctive features of the neuronal cell body. As neurons age, aggregates of a brown pigment...
2.6K
Ribosomal RNA Synthesis
13.1K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.1K
Ribosomes
67.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
67.2K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Neuron Structure
220.4K
Overview
220.4K


