相关实验视频

Updated: Jun 3, 2025

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers
07:18

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

9.5K

脂质双层决定了cAMP与心脏起器通道结合的全ostery,但不是内在的亲和力

Vinay Idikuda, Susovan Roy Chowdhury, Yongchang Chang

    bioRxiv : the preprint server for biology
    |January 7, 2025
    PubMed
    概括

    循环腺单酸盐 (cAMP) 与油脂双层内的HCN离子通道合作结合,与洗剂溶液不同. 这种由脂质诱导的全ostery增强了通道门的灵敏度和节拍活动.

    更多相关视频

    Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers09:54

    Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

    Published on: November 19, 2015

    10.8K
    Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies10:22

    Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

    Published on: July 13, 2013

    19.4K

    相关概念视频

    G-Protein Gated Ion Channels01:21

    G-Protein Gated Ion Channels

    GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
    Sensory...
    4.5K
    Calmodulin-dependent Signaling01:16

    Calmodulin-dependent Signaling

    Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
    The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
    5.1K
    Lipids as Anchors01:32

    Lipids as Anchors

    In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
    The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
    5.5K
    Asymmetric Lipid Bilayer01:35

    Asymmetric Lipid Bilayer

    Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
    7.2K
    Mechanisms of Membrane-bending01:15

    Mechanisms of Membrane-bending

    The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
    Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
    2.6K
    Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

    Ligand-Gated Ion Channel Receptor: Gating Mechanism

    Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
    2.1K