相关实验视频
Updated: Jul 4, 2026

09:09
Pancreatic Islet Embedding for Paraffin Sections
Published on: June 29, 2018
12.7K
长期培养的人类小岛的多omics分析
Guihua Sun1, Meirigeng Qi2, Olivia Sun3
1Department of Neurodegenerative Diseases, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA.
bioRxiv : the preprint server for biology
|January 7, 2025
概括
人类小岛的长期培养揭示了细胞变化和潜在的转化. 这些发现提供了关于胰腺细胞在压力下生存机制的见解,这对于小岛移植成功至关重要.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 贝塔细胞功能障碍是糖尿病进展的主要驱动因素.
- 岛屿移植是糖尿病的一种有前途的治疗策略.
- 了解移植小岛在隔离后发生的细胞变化至关重要.
研究的目的:
- 在长期体外培养 (LTC) 后对人类小岛的特征.
- 为了研究幸存的小岛的分子和细胞变化.
- 探索控制胰腺细胞存活和成熟的机制.
主要方法:
- 人类小岛被长期实验室培养 (LTC).
- 分析了基因表达 (mRNA,miRNA) 和细胞群.
- 评估了细胞特征,包括成熟度标志物.
主要成果:
- 大多数基因是下调的,但在LTC期间,特定的mRNA/miRNA通路是上调的.
- 阿尔法细胞和β细胞分别显示MAFB和MAFA基因表达升高.
- 外分泌细胞被清除的速度比内分泌细胞快;β细胞比α细胞更快.
- 一个不成熟的阿尔法和β细胞种群被丰富,表明可能的转差或脱差.
结论:
- 胰腺细胞具有与成熟和在压力下生存相关的内在机制.
- 在人类小岛上,LTC会诱导显著的细胞和分子变化.
- 这些发现为体外小岛的行为提供了洞察力,这与移植研究有关.
相关概念视频
Methods to Assess Microbial Communities
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
Deep Sea Microbial Ecology
The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
Development of Human Microbiota
The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...

