WhiA转录因子在基因组缩小的细菌中提供翻译和能量生产之间的反循环
Gleb Y Fisunov1, Vladimir B Tsvetkov2,3,4, Ekaterina A Tsoy1
1Scientific Research Institute of Systems Biology and Medicine, Moscow, Russia.
Frontiers in microbiology
|January 7, 2025
概括
菌细胞囊菌WhiA蛋白作为rpsJ操作子的转录抑制剂,感知ATP水平以调节核糖体蛋白质的产生. 它的功能对于结后的生长至关重要.
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- WhiA是细菌中的保存转录因子,特别是在Mollicutes中.
- 它在Mycoplasma gallisepticum中的功能以前是未知的.
- WhiA包括一个DNA结合的HTH域和一个指向内核酶 (HEN) 域.
研究的目的:
- 为了研究来自Mycoplasma gallisepticum的WhiA的DNA结合和ATP结合特性.
- 阐明WhiA在M. gallisepticum中的转录调节作用.
- 了解WhiA介导基因调节的机制.
主要方法:
- 分子动力学模拟的模拟.
- 电泳运动移动性转变试验 (EMSA)
- 微尺度热泳 (MST) 是一种
- 原子力显微镜 (AFM) 的应用
- 基因淘汰研究的研究.
- 记者基因测试试验
- 蛋白质组分析蛋白质组分析
主要成果:
- 在rpsJ操作子的促进子中,WhiA与保存的GAYACRCY核心动机结合.
- WhiA作为rpsJ操作子的转录抑制剂,并充当ATP传感器.
- 无论是HTH和HEN域都对WhiA的抑制器功能至关重要,HEN域与辅助GTTGT动机结合.
- 在冷后,WhiA Knockdown会影响M. gallisepticum的生长,但不会影响活跃生长的细胞.
结论:
- 在M. gallisepticum中,WhiA调节了rpsJ操作子,以响应ATP水平.
- 在低ATP的情况下,WhiA抑制转录;在高ATP的情况下,它抑制转录,增加核糖体蛋白质的产生.
- 在M. gallisepticum中,WhiA在细胞适应压力,特别是结方面发挥着作用.
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