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温西白卵巢在筑巢和晚期产卵阶段的造血功能,组织学特征和转录组概况

Chunfang Zhao¹, Tao Jin², Kefeng Yang²

¹College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, PR China; Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Chuzhou 233100, PR China; Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei 236065, PR China.

Poultry science

|January 7, 2025

概括

这项研究揭示了在放卵停止期间子卵巢的关键分子差异,确定了参与生殖变化的特定基因和lncRNAs,并提供了对鸟类生殖生理学的见解.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
分子内分泌学分子内分泌学

背景情况:

的繁殖停止受到多种因素的影响,但精确的分子机制尚不清楚.
了解这些机制对于改善鸟类生殖效率和管理至关重要.

研究的目的:

为了研究从晚产卵期过渡到筑巢期间的万西白卵巢中的分子和荷尔蒙变化.
识别与生殖停止相关的差异表达的信使RNA (mRNA) 和长非编码RNA (lncRNA).

主要方法:

血液构造参数和血清激素水平 (LH,E2,P4,PRL) 的分析.
RNA测序 (RNA-Seq) 用于在卵巢组织中识别差异表达基因 (DEG) 和 lncRNA (DE lncRNA).
相关性分析和功能丰富分析 (焦点粘附,ECM与受体相互作用,CAMs,PI3K-Akt信号传递).

主要成果:

观察到甘油三,性酸酶,白细胞,中性粒细胞,血红蛋白和血红素水平的显著变化.
荷尔蒙水平 (LH,E2,P4) 增加,而在晚期产卵期间,益生菌素下降.
确定了1,257个DEG和340个DE lncRNA,其中特定的基因和lncRNA与生殖调节有关.
功能丰富分析突出了焦点粘附和ECM受体相互作用等途径.

结论:

特定的DEGs和DE lncRNAs在子的卵巢功能和生殖停止的调节中发挥着关键作用.
荷尔蒙和分子路径的改变是产卵停止期间观察到的生理变化的基础.
这项研究为的生殖变异的分子基础提供了宝贵的见解.

关键词:

血液形成学参数血液形成学参数这是一个LncRNARNA.筑巢在筑巢血清荷尔蒙是一种血清激素.旺西的白是一种白.

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