在Acanthopagrus latus中的全基因组微卫星:发展,分布,表征和多态性
Chao Peng1, Congqiang Luo1, Guangqing Xiang2
1Changde Key Innovation Team for Wetland Biology and Environmental Ecology, Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, College of Life and Environmental Science, Hunan University of Arts and Science, Changde 415000, China.
Animals : an open access journal from MDPI
|January 8, 2025
概括
这项研究在黄海基因组中确定了超过30万个简单序列重复 (SSR),开发了众多SSR标记. 这些标记物显示出高多态性和跨物种实用性,促进了这一重要鱼类的分子育种.
科学领域:
- 基因组学就是基因组学.
- 水产养殖是水产养殖的一种方式.
- 分子生物学分子生物学
背景情况:
- 黄海 (Acanthopagrus latus) 是亚洲的一个关键的水产养殖物种.
- 有限的简单序列重复 (SSR) 数据阻碍了繁殖的进展.
研究的目的:
- 从A. latus基因组中全面隔离和描述SSR.
- 开发和验证用于分子育种和遗传评估的SSR标记物.
主要方法:
- 全基因组隔离和SSRs的表征.
- 对SSR分布,频率和动图类型的生物信息分析.
- 设计SSR标记物,放大,多态性评估和跨物种可转移性测试.
主要成果:
- 确定了318,862种SSR,主要是二核酸 (76.92%) 和三核酸 (15.75%).
- (AC) n图案是最丰富的 (53%).
- 开发了217,791个SSR标记,其中8/9显示高多态性和33/37个位点可转移到A. schlegelii.
结论:
- 这项研究为A. latus.提供了SSR和标记物的基础资源.
- 开发的标记物对分子标记物辅助育种和遗传多样性评估有价值.
- 高多态性和跨物种的可转移性突显了这些SSR的实用性.
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