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过度表达VdCHS2增强了安托西亚宁生物合成,调节了组成比率,并增加了维大卫的细胞中的抗氧化活性

Liyuan He^1,2, Gongti Lai^1,2, Junxuan Lin^1,2

¹Institute of Food Science and Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China.

Antioxidants (Basel, Switzerland)

|January 8, 2025

概括

这项研究开发了一个植物细胞工厂生产安东,通过在葡萄细胞中过度表达VdCHS2基因,实现了60%的产量增加. 这种方法为宝贵的植物化合物提供了传统提取和合成的可持续替代方案.

科学领域:

植物生物技术植物生物技术
代谢工程是代谢工程.
自然产品的合成自然产品的合成

背景情况:

安西氨酸是重要的植物代谢物,在各种行业中具有显著的健康益处和应用.
传统的安托素生产方法面临着资源枯竭和环境污染等挑战.
植物细胞工厂为获得安东素提供了一个可持续和高效的替代方案.

研究的目的:

建立一个植物细胞工厂,使用基因工程来生产高产的安托素.
调查VdCHS2基因过度表达对葡萄细胞中安素生物合成和组成的影响.
阐明了增强的安东素积累背后的分子机制.

主要方法:

用VdCHS2基因对脊柱葡萄细胞进行基因转化.
优化照明条件 (白色和蓝色光) 以促进细胞生长和酸的产生.
有针对性的代谢学分析,以量化安东含量和识别差异积累的代谢物.
基因表达与氨酸生物合成途径有关的基因表达的分析.
评估抗氧化酶活性和活性氧物种水平.

主要成果:

过度表达VdCHS2的OE1细胞系显示,总抗生素含量增加了60% (11 mg/g).
与白光相比,蓝光显著增强了安托素的产生.
过度表达VdCHS2在安东生物合成途径 (CHI,F3Hb,F3'5'H,DFR4,LDOX) 中对关键基因进行上调.
OE1细胞系表现出增强的抗氧化活性和减少氧化应激标志物 (H2O2,MDA).

结论:

VdCHS2基因在增强安东积累和改变葡萄细胞中的组成方面发挥着至关重要的作用.
成功建立了植物细胞工厂模型,以实现高效和可持续的安托素生产.
这种方法为针对性合成安托素成分和开发植物天然产品提供了基础.

关键词:

这就是CHS基因.安托氨酸生物合成抗氧化剂是一种抗氧化剂.这是光谱的光谱.脊柱葡萄 (Vitis davidii Foëx.) 是一种葡萄.

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