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来自人工水系统的Legionella中的抗微生物耐药性基因:为期两年的研究结果

Bernardo Beirão Pereira¹, Mário Marrafa¹, Carolina Cruz¹

¹Laboratory of Microbiology, Nova Medical School, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal.

Antibiotics (Basel, Switzerland)

|January 8, 2025

概括

像lpeAB这样的抗微生物耐药性基因 (ARG) 在军团菌中普遍存在,特别是在L. pneumophila sg1. 监测这些Legionella的ARG对于评估与军团兵相关的公共卫生风险至关重要.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
公共卫生公共卫生

背景情况:

军团菌种引起军团士兵病,并获得抗菌素耐药性基因 (ARG) 由于环境的抗菌素污染.
鉴于潜在的公共卫生风险,评估雷地昂尼拉病毒中ARG的存在至关重要.

研究的目的:

检测和分析Legionella物种中ARG lpeAB和tet56的流行情况.
调查lpeAB和特定的军团菌亚群之间的关联.
为了评估LpeAB对阿兹菌素耐药性的影响.

主要方法:

在348个样本中使用常规PCR检测ARG lpeAB和tet56.
在lpeAB阳性隔离物中评估了阿兹罗密 (AZT) MIC值和LpeAB活性.

主要成果:

在19%的样本中检测到lpeAB基因,在L. pneumophila (30%) 和L. pneumophila sg1 (41%) 中的比率更高.
在lpeAB和L. pneumophila sg1.1之间发现了积极的关联.
阳性lpeAB分离物显示亚齐思罗米MIC为0.064至2mg/L,而LpeAB抑制在大多数分离物中降低了MIC.

结论:

在L. pneumophila sg1.1中,lpeAB基因占主导地位.
一些lpeAB阳性分离物具有MIC值低于野生型菌株的EUCAST初步最高值.
扩大对军团菌的ARG监测对于公共卫生风险评估至关重要.

关键词:

这就是ARG ARG.军团菌 (Legionella) 是一种传染病.流感性肺炎菌 (Legionella pneumophila) 是一种流感性肺炎菌.LpeABAB LpeAB 这是一个很大的问题.这就是为什么Tet56是TET56 抗微生物耐药性抗微生物耐药性

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