转录基因分析揭示了在医院废水中的活跃表达的抗微生物耐药基因及其宿主
Yusuke Ota1, Fei Chen1, Isaac Prah1
1Department of Molecular Microbiology and Immunology, Institute of Science Tokyo, Tokyo 113-8510, Japan.
Antibiotics (Basel, Switzerland)
|January 8, 2025
概括
医院的废水含有抗微生物药物耐药性基因,主要是在质粒上,构成全球健康威胁. 这项研究确定了废水中的关键基因和细菌,以有效监测抗菌素耐药性.
科学领域:
- 环境微生物学环境微生物学
- 基因组学和转录基因组学
- 公共卫生 公共卫生
背景情况:
- 抗菌素耐药性 (AMR) 是一个重大的全球健康和经济挑战.
- 医院的废水是抗菌素耐药生物体和耐药基因的储存库.
- 了解医院废水中的AMR动态对于有效的监测和控制至关重要.
研究的目的:
- 通过综合的元基因组和元转录组分析,阐明医院废水中抗菌素耐药性的动态.
- 识别积极表达的抗微生物耐药性基因和相关细菌.
- 调查塑在抗菌素耐药性传播中的作用.
主要方法:
- 医院废水的综合元基因组和元转录组测序.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于药物检测.
- 组装的基因组和等离子体的生物信息分析.
- 耐药细菌的分离和表型特征.
主要成果:
- 多种药物耐药性基因在染色体和等离子体DNA上均普遍存在,等离子体表现较高 (mRNA/DNA比率).
- 检测到特定药物 (安皮西林,苏尔巴克坦,勒沃素,硫甲醇,trimethoprim),可能导致抗药性.
- 在Serratia nevei中,MAG14表现出AMR基因的高表达,以及相关的多药耐药Serratia sp. 是孤立的. 这是孤立的.
- 等离子体传播的基因被积极表达,表明它们在AMR传播中的重要作用.
结论:
- 医院的废水港口积极表达抗菌素耐药性基因,特别是在质粒上.
- 塞拉提亚内维和相关物种是医院废水AMR的关键参与者.
- 综合的omics方法为AMR动态提供了全面的见解.
- 调查结果强调了医院废水中抗菌素耐药性监测的关键目标,以保护公众和环境健康.
更多相关视频
相关概念视频
01:33Bacterial Transformation
55.1K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
55.1K
00:57Antibiotic Selection
52.2K
Overview
52.2K
00:46Genomic DNA in Prokaryotes
43.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.4K